@article{33dee6d051a611dd8d9f000ea68e967b,
title = "Repression of p15INK4b expression by Myc through association with Miz-1.",
abstract = "Deregulated expression of c-myc can induce cell proliferation in established cell lines and in primary mouse embryonic fibroblasts (MEFs), through a combination of both transcriptional activation and repression by Myc. Here we show that a Myc-associated transcription factor, Miz-1, arrests cells in G1 phase and inhibits cyclin D-associated kinase activity. Miz-1 upregulates expression of the cyclin-dependent kinases (CDK) inhibitor p15INK4b by binding to the initiator element of the p15INK4b promoter. Myc and Max form a complex with Miz-1 at the p15 initiator and inhibit transcriptional activation by Miz-1. Expression of Myc in primary cells inhibits the accumulation of p15INK4b that is associated with cellular senescence; conversely, deletion of c-myc in an established cell line activates p15INK4b expression. Alleles of c-myc that are unable to bind to Miz-1 fail to inhibit accumulation of p15INK4b messenger RNA in primary cells and are, as a consequence, deficient in immortalization.",
author = "P Staller and K Peukert and A Kiermaier and J Seoane and J Lukas and H Karsunky and T M{\"o}r{\"o}y and J Bartek and J Massagu{\'e} and F H{\"a}nel and M Eilers",
note = "Keywords: 3T3 Cells; Animals; Carrier Proteins; Cell Cycle Proteins; Cyclin-Dependent Kinase Inhibitor p15; Cyclin-Dependent Kinase Inhibitor p16; DNA-Binding Proteins; Gene Expression Regulation; Hela Cells; Humans; Kruppel-Like Transcription Factors; Mice; Nuclear Proteins; Promoter Regions (Genetics); Proto-Oncogene Proteins c-myc; Rats; Recombinant Fusion Proteins; Trans-Activators; Transcription Factors; Tumor Suppressor Proteins; Zinc Fingers",
year = "2001",
doi = "10.1038/35070076",
language = "English",
volume = "3",
pages = "392--9",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "nature publishing group",
number = "4",
}