Rad52 and Rad59 exhibit both overlapping and distinct functions

Qi Feng; Louis Düring; Adriana Antúnez de Mayolo; Gaëlle Lettier Buchhorn; Michael Lisby; Naz Erdeniz; Uffe H Mortensen; Rodney Rothstein

doi:10.1016/j.dnarep.2006.08.007

Rad52 and Rad59 exhibit both overlapping and distinct functions

Qi Feng, Louis Düring, Adriana Antúnez de Mayolo, Gaëlle Lettier Buchhorn, Michael Lisby, Naz Erdeniz, Uffe H Mortensen, Rodney Rothstein

Functional genomics

Abstract

Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

Originalsprog	Engelsk
Tidsskrift	DNA Repair
Vol/bind	6
Udgave nummer	1
Sider (fra-til)	27-37
Antal sider	11
ISSN	1568-7864
DOI	https://doi.org/10.1016/j.dnarep.2006.08.007
Status	Udgivet - 2007

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10.1016/j.dnarep.2006.08.007

Citationsformater

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title = "Rad52 and Rad59 exhibit both overlapping and distinct functions",

abstract = "Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.",

author = "Qi Feng and Louis D{\"u}ring and {de Mayolo}, {Adriana Ant{\'u}nez} and Buchhorn, {Ga{\"e}lle Lettier} and Michael Lisby and Naz Erdeniz and Mortensen, {Uffe H} and Rodney Rothstein",

year = "2007",

doi = "10.1016/j.dnarep.2006.08.007",

language = "English",

volume = "6",

pages = "27--37",

journal = "DNA Repair",

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TY - JOUR

T1 - Rad52 and Rad59 exhibit both overlapping and distinct functions

AU - Feng, Qi

AU - Düring, Louis

AU - de Mayolo, Adriana Antúnez

AU - Buchhorn, Gaëlle Lettier

AU - Lisby, Michael

AU - Erdeniz, Naz

AU - Mortensen, Uffe H

AU - Rothstein, Rodney

PY - 2007

Y1 - 2007

N2 - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

AB - Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.

U2 - 10.1016/j.dnarep.2006.08.007

DO - 10.1016/j.dnarep.2006.08.007

M3 - Journal article

SN - 1568-7864

VL - 6

SP - 27

EP - 37

JO - DNA Repair

JF - DNA Repair

IS - 1

ER -

Rad52 and Rad59 exhibit both overlapping and distinct functions

Abstract

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