Protein feature based identification of cell cycle regulated proteins in yeast

TY - JOUR

T1 - Protein feature based identification of cell cycle regulated proteins in yeast

AU - de Lichtenberg, Ulrik

AU - Jensen, Thomas S

AU - Jensen, Lars J

AU - Brunak, Søren

PY - 2003

Y1 - 2003

N2 - DNA microarrays have been used extensively to identify cell cycle regulated genes in yeast; however, the overlap in the genes identified is surprisingly small. We show that certain protein features can be used to distinguish cell cycle regulated genes from other genes with high confidence (features include protein phosphorylation, glycosylation, subcellular location and instability/degradation). We demonstrate that co-expressed, periodic genes encode proteins which share combinations of features, and provide an overview of the proteome dynamics during the cycle. A large set of novel putative cell cycle regulated proteins were identified, many of which have no known function.

AB - DNA microarrays have been used extensively to identify cell cycle regulated genes in yeast; however, the overlap in the genes identified is surprisingly small. We show that certain protein features can be used to distinguish cell cycle regulated genes from other genes with high confidence (features include protein phosphorylation, glycosylation, subcellular location and instability/degradation). We demonstrate that co-expressed, periodic genes encode proteins which share combinations of features, and provide an overview of the proteome dynamics during the cycle. A large set of novel putative cell cycle regulated proteins were identified, many of which have no known function.

M3 - Journal article

SN - 0022-2836

VL - 329

SP - 663

EP - 674

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -