Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates

Ulrik Lausten-Thomsen; Hans Ole Madsen; Therese Risom Vestergaard; Henrik Hjalgrim; Jacob Nersting; Kjeld Schmiegelow

doi:10.1182/blood-2010-05-282764

Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates

Ulrik Lausten-Thomsen, Hans Ole Madsen, Therese Risom Vestergaard, Henrik Hjalgrim, Jacob Nersting, Kjeld Schmiegelow

59 Citationer (Scopus)

Abstract

t(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood samples were isolated within 24 hours from birth and subsequently screened for ETV6-RUNX1 transcripts using a highly sensitive real-time reverse transcription polymerase chain reaction assay. In first-run polymerase chain reaction, 14 samples were positive at levels below 10(-5), of which specific hybridization reflecting the relevant genetic region was positive in 9 cases. Repeated analyses using stored mRNA and flowcytometric sorting of a CD19(+), CD8(+), and CD19(-)/CD8(-) subpopulations from cryopreserved mononuclear cells from the same cord blood samples (mean sorted: 18 × 10(6) cells) revealed no positive findings, which demonstrates that the level and/or frequency of ETV6-RUNX1-positive cells is markedly lower than suggested in previous studies.

Originalsprog	Engelsk
Tidsskrift	Blood
Vol/bind	117
Udgave nummer	1
Sider (fra-til)	186-9
Antal sider	4
ISSN	0006-4971
DOI	https://doi.org/10.1182/blood-2010-05-282764
Status	Udgivet - 6 jan. 2011

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10.1182/blood-2010-05-282764

https://ashpublications.org/blood/article-pdf/117/1/186/1335231/zh800111000186.pdf

Citationsformater

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title = "Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates",

abstract = "t(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood samples were isolated within 24 hours from birth and subsequently screened for ETV6-RUNX1 transcripts using a highly sensitive real-time reverse transcription polymerase chain reaction assay. In first-run polymerase chain reaction, 14 samples were positive at levels below 10(-5), of which specific hybridization reflecting the relevant genetic region was positive in 9 cases. Repeated analyses using stored mRNA and flowcytometric sorting of a CD19(+), CD8(+), and CD19(-)/CD8(-) subpopulations from cryopreserved mononuclear cells from the same cord blood samples (mean sorted: 18 × 10(6) cells) revealed no positive findings, which demonstrates that the level and/or frequency of ETV6-RUNX1-positive cells is markedly lower than suggested in previous studies.",

author = "Ulrik Lausten-Thomsen and Madsen, {Hans Ole} and Vestergaard, {Therese Risom} and Henrik Hjalgrim and Jacob Nersting and Kjeld Schmiegelow",

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doi = "10.1182/blood-2010-05-282764",

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T1 - Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates

AU - Lausten-Thomsen, Ulrik

AU - Madsen, Hans Ole

AU - Vestergaard, Therese Risom

AU - Hjalgrim, Henrik

AU - Nersting, Jacob

AU - Schmiegelow, Kjeld

PY - 2011/1/6

Y1 - 2011/1/6

N2 - t(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood samples were isolated within 24 hours from birth and subsequently screened for ETV6-RUNX1 transcripts using a highly sensitive real-time reverse transcription polymerase chain reaction assay. In first-run polymerase chain reaction, 14 samples were positive at levels below 10(-5), of which specific hybridization reflecting the relevant genetic region was positive in 9 cases. Repeated analyses using stored mRNA and flowcytometric sorting of a CD19(+), CD8(+), and CD19(-)/CD8(-) subpopulations from cryopreserved mononuclear cells from the same cord blood samples (mean sorted: 18 × 10(6) cells) revealed no positive findings, which demonstrates that the level and/or frequency of ETV6-RUNX1-positive cells is markedly lower than suggested in previous studies.

AB - t(12;21)(p13;q22)[ETV6-RUNX1] is the most common chromosomal translocation in childhood acute lymphoblastic leukemia, and it can often be backtracked to Guthrie cards supporting prenatal initiation and high levels of circulating t(12;21)-positive cells at birth. To explore the prevalence of ETV6-RUNX1-positive cells in healthy neonates, mononuclear cells from 1417 umbilical cord blood samples were isolated within 24 hours from birth and subsequently screened for ETV6-RUNX1 transcripts using a highly sensitive real-time reverse transcription polymerase chain reaction assay. In first-run polymerase chain reaction, 14 samples were positive at levels below 10(-5), of which specific hybridization reflecting the relevant genetic region was positive in 9 cases. Repeated analyses using stored mRNA and flowcytometric sorting of a CD19(+), CD8(+), and CD19(-)/CD8(-) subpopulations from cryopreserved mononuclear cells from the same cord blood samples (mean sorted: 18 × 10(6) cells) revealed no positive findings, which demonstrates that the level and/or frequency of ETV6-RUNX1-positive cells is markedly lower than suggested in previous studies.

U2 - 10.1182/blood-2010-05-282764

DO - 10.1182/blood-2010-05-282764

M3 - Journal article

SN - 0006-4971

VL - 117

SP - 186

EP - 189

JO - Blood

JF - Blood

IS - 1

ER -

Prevalence of t(12;21)[ETV6-RUNX1]-positive cells in healthy neonates

Abstract

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