Photosystem II assembly from scratch

Thilo Rühle; Dario Michael Leister

doi:10.3389/fpls.2015.01234

Photosystem II assembly from scratch

Thilo Rühle, Dario Michael Leister

Molekylær Plantebiologi

2 Citationer (Scopus)

33 Downloads (Pure)

Abstract

Construction of a functional PhotosystemII (PSII) in cyanobacteria and chloroplasts depends on the action of auxiliary factors, which transiently interact with PSII intermediates during assembly. In addition to a common PSII structure and a conserved set of PSII assembly factors, cyanobacteria, and higher plants have evolved additional, clade-specific assembly factors. Most such factors in cyanobacteria and chloroplasts have been identified by “top-down” approaches (forward and reverse genetics), which involved genetic disruption of individual components in the assembly process and subsequent characterization of the ensuing phenotypic effects on the respective mutantlines/strains. In contrast, a “bottom-up” strategy, based on the engineering of a synthetic bacterium with a plant-type PSII, has the potential to identify all assembly factors sufficient to make a functional plant PSII.

Originalsprog	Engelsk
Artikelnummer	1234
Tidsskrift	Frontiers in Plant Science
Vol/bind	6
Antal sider	5
ISSN	1664-462X
DOI	https://doi.org/10.3389/fpls.2015.01234
Status	Udgivet - 12 jan. 2016

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10.3389/fpls.2015.01234

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AU - Leister, Dario Michael

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AB - Construction of a functional PhotosystemII (PSII) in cyanobacteria and chloroplasts depends on the action of auxiliary factors, which transiently interact with PSII intermediates during assembly. In addition to a common PSII structure and a conserved set of PSII assembly factors, cyanobacteria, and higher plants have evolved additional, clade-specific assembly factors. Most such factors in cyanobacteria and chloroplasts have been identified by “top-down” approaches (forward and reverse genetics), which involved genetic disruption of individual components in the assembly process and subsequent characterization of the ensuing phenotypic effects on the respective mutantlines/strains. In contrast, a “bottom-up” strategy, based on the engineering of a synthetic bacterium with a plant-type PSII, has the potential to identify all assembly factors sufficient to make a functional plant PSII.

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Photosystem II assembly from scratch

Abstract

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