Optimal developmental stage for vitrification of parthenogenetically activated porcine embryos

TY - JOUR

T1 - Optimal developmental stage for vitrification of parthenogenetically activated porcine embryos

AU - Li, Rong

AU - Li, Juan

AU - Kragh, Peter

AU - Hyttel, Poul

AU - Schmidt, Mette

AU - Callesen, Henrik

PY - 2012/2

Y1 - 2012/2

N2 - The objective of this experiment was to determine the optimal developmental stage to vitrify in vitro cultured porcine parthenogenetically activated (PA) embryos. Embryos were vitrified by Cryotop on Day 4, 5 or 6 after oocyte activation (Day 0), and immediately after warming they were either time-lapse monitored for 24. h or analyzed by differential staining. After warming, the embryos had to be cultured for at least 8. h before their survival rates were stabilized. Both the survival rate at 8. h and the hatching rate at 24. h of Day 4 embryos were significant higher than those vitrified on Day 5 or 6 (P<0.05), no matter if they were morulae or blastocysts. These results demonstrate that porcine PA embryos can survive successfully after vitrification/warming, that the optimal time for vitrification was Day 4 for both morulae and blastocysts, and that 8. h after warming was the time needed to make an early evaluation of porcine PA embryo survival.

AB - The objective of this experiment was to determine the optimal developmental stage to vitrify in vitro cultured porcine parthenogenetically activated (PA) embryos. Embryos were vitrified by Cryotop on Day 4, 5 or 6 after oocyte activation (Day 0), and immediately after warming they were either time-lapse monitored for 24. h or analyzed by differential staining. After warming, the embryos had to be cultured for at least 8. h before their survival rates were stabilized. Both the survival rate at 8. h and the hatching rate at 24. h of Day 4 embryos were significant higher than those vitrified on Day 5 or 6 (P<0.05), no matter if they were morulae or blastocysts. These results demonstrate that porcine PA embryos can survive successfully after vitrification/warming, that the optimal time for vitrification was Day 4 for both morulae and blastocysts, and that 8. h after warming was the time needed to make an early evaluation of porcine PA embryo survival.

U2 - 10.1016/j.cryobiol.2011.08.008

DO - 10.1016/j.cryobiol.2011.08.008

M3 - Journal article

SN - 0011-2240

VL - 64

SP - 60

EP - 64

JO - Cryobiology

JF - Cryobiology

IS - 1

ER -