TY - JOUR
T1 - On the regulatory functions of neuropeptide Y (NPY) with respect to vascular resistance and exocrine and endocrine secretion in the pig pancreas
AU - Holst, J J
AU - Orskov, C
AU - Knuhtsen, S
AU - Sheikh, S
AU - Nielsen, O V
PY - 1989/8
Y1 - 1989/8
N2 - We compared the effects of electrical stimulation of the splanchnic nerves and infusions of neuropeptide Y, noradrenaline or a combination of the two on pancreatic vascular resistance and exocrine and endocrine secretion. For these studies we used isolated perfused pig pancreas with preserved splanchnic nerve supply. The exocrine secretion was stimulated with physiological concentrations of secretin and cholecystokinin octapeptide. Noradrenaline and NPY at 10(-8) M both increased pancreatic perfusion pressure. Their effects were additive and similar in magnitude to that of electrical stimulation of the splanchnic nerves at 4-8 Hz. Nerve stimulation as well as NPY and noradrenaline infusions inhibited exocrine secretion, but an additive effect could not be demonstrated. Neither NPY nor noradrenaline could reproduce the stimulatory effect of nerve stimulation on glucagon secretion, nor the weak inhibitory effect on somatostatin secretion. NPY alone had no effect on insulin secretion and did not influence the inhibitory effect of noradrenaline. It is concluded that NPY is likely to cooperate with noradrenaline in the control of pancreatic blood flow, whereas its role in the control of pancreatic secretion is likely to be of minor importance, if any.
AB - We compared the effects of electrical stimulation of the splanchnic nerves and infusions of neuropeptide Y, noradrenaline or a combination of the two on pancreatic vascular resistance and exocrine and endocrine secretion. For these studies we used isolated perfused pig pancreas with preserved splanchnic nerve supply. The exocrine secretion was stimulated with physiological concentrations of secretin and cholecystokinin octapeptide. Noradrenaline and NPY at 10(-8) M both increased pancreatic perfusion pressure. Their effects were additive and similar in magnitude to that of electrical stimulation of the splanchnic nerves at 4-8 Hz. Nerve stimulation as well as NPY and noradrenaline infusions inhibited exocrine secretion, but an additive effect could not be demonstrated. Neither NPY nor noradrenaline could reproduce the stimulatory effect of nerve stimulation on glucagon secretion, nor the weak inhibitory effect on somatostatin secretion. NPY alone had no effect on insulin secretion and did not influence the inhibitory effect of noradrenaline. It is concluded that NPY is likely to cooperate with noradrenaline in the control of pancreatic blood flow, whereas its role in the control of pancreatic secretion is likely to be of minor importance, if any.
KW - Animals
KW - Cholecystokinin/administration & dosage
KW - Dose-Response Relationship, Drug
KW - Electric Stimulation
KW - Infusions, Intravenous
KW - Islets of Langerhans/drug effects
KW - Neuropeptide Y/administration & dosage
KW - Norepinephrine/administration & dosage
KW - Pancreas/drug effects
KW - Secretin/administration & dosage
KW - Splanchnic Nerves/physiology
KW - Swine
KW - Vascular Resistance/drug effects
U2 - 10.1111/j.1748-1716.1989.tb08697.x
DO - 10.1111/j.1748-1716.1989.tb08697.x
M3 - Journal article
C2 - 2675536
SN - 0001-6772
VL - 136
SP - 519
EP - 526
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
IS - 4
ER -