Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology

Jan Stenvang Jepsen; Mads D Sørensen; Jesper Wengel; Jan Stenvang

doi:10.1089/1545457041526317

Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology

Jan Stenvang Jepsen, Mads D Sørensen, Jesper Wengel, Jan Stenvang

148 Citationer (Scopus)

Abstract

Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.

Originalsprog	Engelsk
Tidsskrift	Oligonucleotides
Vol/bind	14
Udgave nummer	2
Sider (fra-til)	130-46
Antal sider	17
ISSN	1545-4576
DOI	https://doi.org/10.1089/1545457041526317
Status	Udgivet - 2004

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10.1089/1545457041526317

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abstract = "Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.",

keywords = "Animals, Biotechnology, Drug Design, Humans, Oligonucleotides, Oligonucleotides, Antisense, Ribonuclease H",

author = "Jepsen, {Jan Stenvang} and S{\o}rensen, {Mads D} and Jesper Wengel and Jan Stenvang",

year = "2004",

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T1 - Locked nucleic acid

T2 - a potent nucleic acid analog in therapeutics and biotechnology

AU - Jepsen, Jan Stenvang

AU - Sørensen, Mads D

AU - Wengel, Jesper

AU - Stenvang, Jan

PY - 2004

Y1 - 2004

N2 - Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.

AB - Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.

KW - Animals

KW - Biotechnology

KW - Drug Design

KW - Humans

KW - Oligonucleotides

KW - Oligonucleotides, Antisense

KW - Ribonuclease H

U2 - 10.1089/1545457041526317

DO - 10.1089/1545457041526317

M3 - Journal article

C2 - 15294076

SN - 2159-3337

VL - 14

SP - 130

EP - 146

JO - Nucleic Acid Therapeutics

JF - Nucleic Acid Therapeutics

IS - 2

ER -

Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology

Abstract

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