Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

Thomas Kaasgaard; Ole G. Mouritsen; Kent Jorgensen

doi:10.1016/S0014-5793(02)02391-8

Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

Thomas Kaasgaard, Ole G. Mouritsen, Kent Jorgensen^*

^*Corresponding author af dette arbejde

23 Citationer (Scopus)

Abstract

A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	515
Udgave nummer	1-3
Sider (fra-til)	29-34
Antal sider	6
ISSN	0014-5793
DOI	https://doi.org/10.1016/S0014-5793(02)02391-8
Status	Udgivet - 27 mar. 2002

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title = "Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy",

abstract = "A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.",

keywords = "Atomic force microscopy, Avidin, Biotin, Langmuir-Blodgett film, Lipid bilayer membrane, Lipid domain, Lipid membrane heterogeneity, Lipid mixture, Phosphatidylcholine",

author = "Thomas Kaasgaard and Mouritsen, {Ole G.} and Kent Jorgensen",

year = "2002",

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doi = "10.1016/S0014-5793(02)02391-8",

language = "English",

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TY - JOUR

T1 - Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

AU - Kaasgaard, Thomas

AU - Mouritsen, Ole G.

AU - Jorgensen, Kent

PY - 2002/3/27

Y1 - 2002/3/27

N2 - A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.

AB - A novel experimental technique, based on atomic force microscopy (AFM), is proposed to visualize the lateral organization of membrane systems in the nanometer range. The technique involves the use of a ligand-receptor pair, biotin-avidin, which introduces a height variation on a solid-supported lipid bilayer membrane. This leads to a height amplification of the lateral membrane organization that is large enough to be clearly imaged by scanning AFM. The power of the technique is demonstrated for a binary dipalmitoylphosphocholine-diarachidoylphosphocholine lipid mixture which is shown to exhibit a distinct lateral lipid domain formation. The new and simple ligand-receptor-based AFM approach opens up new ways to investigate lipid membrane microstructure in the nanometer range as well as the lateral distribution of ligand-lipid and receptor-protein complexes in supported membrane systems.

KW - Atomic force microscopy

KW - Avidin

KW - Biotin

KW - Langmuir-Blodgett film

KW - Lipid bilayer membrane

KW - Lipid domain

KW - Lipid membrane heterogeneity

KW - Lipid mixture

KW - Phosphatidylcholine

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U2 - 10.1016/S0014-5793(02)02391-8

DO - 10.1016/S0014-5793(02)02391-8

M3 - Journal article

C2 - 11943189

AN - SCOPUS:0037181461

SN - 0014-5793

VL - 515

SP - 29

EP - 34

JO - F E B S Letters

JF - F E B S Letters

IS - 1-3

ER -

Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy

Abstract

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