Isolation and characterization of node/notochord-like cells from mouse embryonic stem cells

Maria K. Winzi; Poul Hyttel; Jacqueline Kim Dale; Palle Serup

doi:10.1089/scd.2011.0042

Isolation and characterization of node/notochord-like cells from mouse embryonic stem cells

Maria K. Winzi, Poul Hyttel, Jacqueline Kim Dale, Palle Serup

13 Citationer (Scopus)

Abstract

The homeobox gene Noto is expressed in the node and its derivative the notochord. Here we use a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. We find very few Noto-expressing cells after spontaneous differentiation. However, the number of Noto-expressing cells was increased when using Activin A to induce a Foxa2- and Brachyury-expressing progenitor population, whose further differentiation into Noto-expressing cells was improved by simultaneous inhibition of BMP, Wnt, and retinoic acid signaling. Noto-GFP(+) cells expressed the node/notochord markers Noto, Foxa2, Shh, Noggin, Chordin, Foxj1, and Brachyury; showed a vacuolarization characteristic of notochord cells; and can integrate into midline structures when grafted into Hensen's node of gastrulating chicken embryos. The ability to generate node/notochord-like cells in vitro will aid the biochemical characterization of these developmentally important structures.

Originalsprog	Engelsk
Tidsskrift	Stem Cells and Development
Vol/bind	20
Udgave nummer	11
Sider (fra-til)	1817-1827
Antal sider	11
ISSN	1547-3287
DOI	https://doi.org/10.1089/scd.2011.0042
Status	Udgivet - nov. 2011

Adgang til dokumentet

10.1089/scd.2011.0042

Citationsformater

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title = "Isolation and characterization of node/notochord-like cells from mouse embryonic stem cells",

abstract = "The homeobox gene Noto is expressed in the node and its derivative the notochord. Here we use a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. We find very few Noto-expressing cells after spontaneous differentiation. However, the number of Noto-expressing cells was increased when using Activin A to induce a Foxa2- and Brachyury-expressing progenitor population, whose further differentiation into Noto-expressing cells was improved by simultaneous inhibition of BMP, Wnt, and retinoic acid signaling. Noto-GFP(+) cells expressed the node/notochord markers Noto, Foxa2, Shh, Noggin, Chordin, Foxj1, and Brachyury; showed a vacuolarization characteristic of notochord cells; and can integrate into midline structures when grafted into Hensen's node of gastrulating chicken embryos. The ability to generate node/notochord-like cells in vitro will aid the biochemical characterization of these developmentally important structures.",

author = "Winzi, {Maria K.} and Poul Hyttel and Dale, {Jacqueline Kim} and Palle Serup",

year = "2011",

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doi = "10.1089/scd.2011.0042",

language = "English",

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T1 - Isolation and characterization of node/notochord-like cells from mouse embryonic stem cells

AU - Winzi, Maria K.

AU - Hyttel, Poul

AU - Dale, Jacqueline Kim

AU - Serup, Palle

PY - 2011/11

Y1 - 2011/11

N2 - The homeobox gene Noto is expressed in the node and its derivative the notochord. Here we use a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. We find very few Noto-expressing cells after spontaneous differentiation. However, the number of Noto-expressing cells was increased when using Activin A to induce a Foxa2- and Brachyury-expressing progenitor population, whose further differentiation into Noto-expressing cells was improved by simultaneous inhibition of BMP, Wnt, and retinoic acid signaling. Noto-GFP(+) cells expressed the node/notochord markers Noto, Foxa2, Shh, Noggin, Chordin, Foxj1, and Brachyury; showed a vacuolarization characteristic of notochord cells; and can integrate into midline structures when grafted into Hensen's node of gastrulating chicken embryos. The ability to generate node/notochord-like cells in vitro will aid the biochemical characterization of these developmentally important structures.

AB - The homeobox gene Noto is expressed in the node and its derivative the notochord. Here we use a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. We find very few Noto-expressing cells after spontaneous differentiation. However, the number of Noto-expressing cells was increased when using Activin A to induce a Foxa2- and Brachyury-expressing progenitor population, whose further differentiation into Noto-expressing cells was improved by simultaneous inhibition of BMP, Wnt, and retinoic acid signaling. Noto-GFP(+) cells expressed the node/notochord markers Noto, Foxa2, Shh, Noggin, Chordin, Foxj1, and Brachyury; showed a vacuolarization characteristic of notochord cells; and can integrate into midline structures when grafted into Hensen's node of gastrulating chicken embryos. The ability to generate node/notochord-like cells in vitro will aid the biochemical characterization of these developmentally important structures.

U2 - 10.1089/scd.2011.0042

DO - 10.1089/scd.2011.0042

M3 - Journal article

C2 - 21351873

SN - 1547-3287

VL - 20

SP - 1817

EP - 1827

JO - Stem Cells and Development

JF - Stem Cells and Development

IS - 11

ER -