Intrinsic reaction-cycle time scale of Na+,K+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Hélène Bouvrais; Flemming Cornelius; John H. Ipsen; Ole G. Mouritsen

doi:10.1073/pnas.1209909109

Intrinsic reaction-cycle time scale of Na⁺,K⁺-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Hélène Bouvrais, Flemming Cornelius, John H. Ipsen, Ole G. Mouritsen^*

^*Corresponding author af dette arbejde

28 Citationer (Scopus)

Abstract

Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually infl uence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na⁺,K⁺-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, τ, which is robust and independent of the speci fi c fl uctuation modes of the membrane. In the case of pumping Na⁺-ions, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≃ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Sciences of the United States of America
Vol/bind	109
Udgave nummer	45
Sider (fra-til)	18442-18446
Antal sider	5
ISSN	0027-8424
DOI	https://doi.org/10.1073/pnas.1209909109
Status	Udgivet - 6 nov. 2012

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10.1073/pnas.1209909109

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Citationsformater

Intrinsic reaction-cycle time scale of Na⁺,K⁺-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes. / Bouvrais, Hélène; Cornelius, Flemming; Ipsen, John H. et al.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 109, Nr. 45, 06.11.2012, s. 18442-18446.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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