In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

John Fleng Steffensen

In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

Marine Biological Section

Abstract

Soc for experimental Biol Annual Meeting - Salzburg 2012

Morten Bo S. Svendsen (University of Copenhagen, Denmark) and
John F. Steffensen (University of Copenhagen, Denmark)

By injecting 1 IJm microspheres containing an oxygen-dependent
phosphorescent dye into the vascular system and tissue of the transparent
glass catfish (Kryptopterus bichirris), it is possible to measure near realtime
oxygen partial pressure in vivo. We used a commercially-available
digital single-lens reflex camera mounted with an optical long pass filter
(II = 490 nm) and excited the phosphorescent dye in the microspheres
inside the fish with externally-mounted blue light emitting diodes
(lip = 470 nm) to image the oxygen partial pressure. This method makes
it possible to investigate oxygen partial pressures in the vascular system
and different tissues of fish without having to insert any probes into the
animal.
After injection of the microspheres and a recovery period from the
anaesthesia, in vivo oxygen partial pressure can be determined by just
taking a picture of the live fish exposed to blue light. As no electrodes or
sensors are attached, the method allows a wide range of experiments
investigating in vivo oxygen levels under different environmental
perturbations.
This poster will show results and method.
Email addressforcorrespondence: [email protected]

Originalsprog	Engelsk
Publikationsdato	2012
Status	Udgivet - 2012

Citationsformater

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title = "In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)",

abstract = "Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: [email protected]",

author = "Steffensen, {John Fleng}",

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TY - ABST

T1 - In vivo near real time imaging of oxygen partial pressures in the glass catfish (Kryptopterus bichirris)

AU - Steffensen, John Fleng

PY - 2012

Y1 - 2012

N2 - Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: [email protected]

AB - Soc for experimental Biol Annual Meeting - Salzburg 2012Morten Bo S. Svendsen (University of Copenhagen, Denmark) andJohn F. Steffensen (University of Copenhagen, Denmark)By injecting 1 IJm microspheres containing an oxygen-dependentphosphorescent dye into the vascular system and tissue of the transparentglass catfish (Kryptopterus bichirris), it is possible to measure near realtimeoxygen partial pressure in vivo. We used a commercially-availabledigital single-lens reflex camera mounted with an optical long pass filter(II = 490 nm) and excited the phosphorescent dye in the microspheresinside the fish with externally-mounted blue light emitting diodes(lip = 470 nm) to image the oxygen partial pressure. This method makesit possible to investigate oxygen partial pressures in the vascular systemand different tissues of fish without having to insert any probes into theanimal.After injection of the microspheres and a recovery period from theanaesthesia, in vivo oxygen partial pressure can be determined by justtaking a picture of the live fish exposed to blue light. As no electrodes orsensors are attached, the method allows a wide range of experimentsinvestigating in vivo oxygen levels under different environmentalperturbations.This poster will show results and method.Email addressforcorrespondence: [email protected]

M3 - Conference abstract for conference

ER -