Hypoxia-induced retinopathy model in adult zebrafish

Ziquan Cao; Lasse D. Jensen; Pegah Rouhi; Kayoko Hosaka; Toste Lanne; John Fleng Steffensen; Erik Wahlberg; Yihai Cao

doi:10.1038/nprot.2010.149

Hypoxia-induced retinopathy model in adult zebrafish

Ziquan Cao, Lasse D. Jensen, Pegah Rouhi, Kayoko Hosaka, Toste Lanne, John Fleng Steffensen, Erik Wahlberg, Yihai Cao

Marine Biological Section

56 Citationer (Scopus)

Abstract

Abstract: Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.

Originalsprog	Engelsk
Tidsskrift	Nature Protocols (Print)
Vol/bind	5
Udgave nummer	12
Sider (fra-til)	1903-1910
Antal sider	8
ISSN	1754-2189
DOI	https://doi.org/10.1038/nprot.2010.149
Status	Udgivet - nov. 2010

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10.1038/nprot.2010.149

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title = "Hypoxia-induced retinopathy model in adult zebrafish",

abstract = "Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.",

author = "Ziquan Cao and Jensen, {Lasse D.} and Pegah Rouhi and Kayoko Hosaka and Toste Lanne and Steffensen, {John Fleng} and Erik Wahlberg and Yihai Cao",

year = "2010",

month = nov,

doi = "10.1038/nprot.2010.149",

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AU - Cao, Ziquan

AU - Jensen, Lasse D.

AU - Rouhi, Pegah

AU - Hosaka, Kayoko

AU - Lanne, Toste

AU - Steffensen, John Fleng

AU - Wahlberg, Erik

AU - Cao, Yihai

PY - 2010/11

Y1 - 2010/11

N2 - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.

AB - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.

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DO - 10.1038/nprot.2010.149

M3 - Journal article

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SN - 1754-2189

VL - 5

SP - 1903

EP - 1910

JO - Nature Protocols

JF - Nature Protocols

IS - 12

ER -

Hypoxia-induced retinopathy model in adult zebrafish

Abstract

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