Hypoxia-induced metastasis model in embryonic zebrafish

TY - JOUR

T1 - Hypoxia-induced metastasis model in embryonic zebrafish

AU - Rouhi, Pegah

AU - Jensen, Lasse D.

AU - Cao, Ziquan

AU - Hosaka, Kayoko

AU - Lanne, Toste

AU - Wahlberg, Eric

AU - Steffensen, John Fleng

AU - Cao, Yihai

PY - 2010/11

Y1 - 2010/11

N2 - Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.

AB - Hypoxia facilitates tumor invasion and metastasis by promoting neovascularization and co-option of tumor cells in the peritumoral vasculature, leading to dissemination of tumor cells into the circulation. However, until recently, animal models and imaging technology did not enable monitoring of the early events of tumor cell invasion and dissemination in living animals. We recently developed a zebrafish metastasis model to dissect the detailed events of hypoxia-induced tumor cell invasion and metastasis in association with angiogenesis at the single-cell level. In this model, fluorescent DiI-labeled human or mouse tumor cells are implanted into the perivitelline cavity of 48-h-old zebrafish embryos, which are subsequently placed in hypoxic water for 3 d. Tumor cell invasion, metastasis and pathological angiogenesis are detected under fluorescent microscopy in the living fish. The average experimental time for this model is 7 d. Our protocol offers a remarkable opportunity to study molecular mechanisms of hypoxia-induced cancer metastasis.

U2 - 10.1038/nprot.2010.150

DO - 10.1038/nprot.2010.150

M3 - Journal article

C2 - 21127485

SN - 1754-2189

VL - 5

SP - 1911

EP - 1918

JO - Nature Protocols

JF - Nature Protocols

IS - 12

ER -