HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity

Wolfram H. Gerlich; Franz F. Wagner; Michael Chudy; Lene Holm Harritshoj; Annette Lattermann; Sandra Wienzek; Dieter Glebe; Mona Saniewski; Christian G. Schüttler; Ulrike C. Wend; Wulf R. Willems; Ursula Bauerfeind; Christine Jork; Gregor Bein; Per Platz; Henrik Ullum; Ebbe Dickmeiss

doi:10.1002/jmv.20963

HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity

Wolfram H. Gerlich^*, Franz F. Wagner, Michael Chudy, Lene Holm Harritshoj, Annette Lattermann, Sandra Wienzek, Dieter Glebe, Mona Saniewski, Christian G. Schüttler, Ulrike C. Wend, Wulf R. Willems, Ursula Bauerfeind, Christine Jork, Gregor Bein, Per Platz, Henrik Ullum, Ebbe Dickmeiss

^*Corresponding author af dette arbejde

47 Citationer (Scopus)

Abstract

Five blood donors with occult persistent and one donor with an early window phase HBV infection were identified. They were negative in regular HBsAg screening and had low levels of HBV DNA that were probably not detectable by current mini-pool nucleic acid amplification testing. In four donors several mutations were found located in the HBs antigen loop. In three donors the mutations were predominantly outside the "a" determinant; one donor had a wild type HBsAg sequence. Fifty-five recipients of donations from the persistently infected donors could be tested for previous or ongoing HBV infection and of them 53% (29/55) were anti-HBc positive. Based on the prevalence of anti-HBc in Germany (7%), it was assumed that four of those recipients had already been positive before transfusion. In 22 cases, it was assumed that they acquired infection by the donations, but the infection remained asymptomatic and was resolved. In three cases transmission was proven by the time course of the acute infection and sequence identity The resulting infection was fatal and associated with immunological disorders at the time of transmission: in one case sepsis and in the other two cases immunosuppression. In a further asymptomatic case of proven transmission from the early window phase donation passively administered anti-HBs could not prevent spread of wildtype HBV but antiviral treatment lead to resolution. Surprisingly the typical acute hepatitis B was not observed in a single one of the 26 cases of assumed or proven transmission.

Originalsprog	Engelsk
Tidsskrift	Journal of Medical Virology
Vol/bind	79
Udgave nummer	SUPPL. 1
ISSN	0146-6615
DOI	https://doi.org/10.1002/jmv.20963
Status	Udgivet - 1 okt. 2007

FN’s Verdensmål

Dette resultat bidrager til følgende verdensmål

Adgang til dokumentet

10.1002/jmv.20963

Andre filer og links

Link to publication in Scopus

Citationsformater

Gerlich, W. H., Wagner, F. F., Chudy, M., Harritshoj, L. H., Lattermann, A., Wienzek, S., Glebe, D., Saniewski, M., Schüttler, C. G., Wend, U. C., Willems, W. R., Bauerfeind, U., Jork, C., Bein, G., Platz, P., Ullum, H., & Dickmeiss, E. (2007). HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity. Journal of Medical Virology, 79(SUPPL. 1). https://doi.org/10.1002/jmv.20963

Gerlich, WH, Wagner, FF, Chudy, M, Harritshoj, LH, Lattermann, A, Wienzek, S, Glebe, D, Saniewski, M, Schüttler, CG, Wend, UC, Willems, WR, Bauerfeind, U, Jork, C, Bein, G, Platz, P, Ullum, H & Dickmeiss, E 2007, 'HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity', Journal of Medical Virology, bind 79, nr. SUPPL. 1. https://doi.org/10.1002/jmv.20963

@article{cbe1aeeda1df4970afdda1312be175be,

title = "HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity",

abstract = "Five blood donors with occult persistent and one donor with an early window phase HBV infection were identified. They were negative in regular HBsAg screening and had low levels of HBV DNA that were probably not detectable by current mini-pool nucleic acid amplification testing. In four donors several mutations were found located in the HBs antigen loop. In three donors the mutations were predominantly outside the {"}a{"} determinant; one donor had a wild type HBsAg sequence. Fifty-five recipients of donations from the persistently infected donors could be tested for previous or ongoing HBV infection and of them 53% (29/55) were anti-HBc positive. Based on the prevalence of anti-HBc in Germany (7%), it was assumed that four of those recipients had already been positive before transfusion. In 22 cases, it was assumed that they acquired infection by the donations, but the infection remained asymptomatic and was resolved. In three cases transmission was proven by the time course of the acute infection and sequence identity The resulting infection was fatal and associated with immunological disorders at the time of transmission: in one case sepsis and in the other two cases immunosuppression. In a further asymptomatic case of proven transmission from the early window phase donation passively administered anti-HBs could not prevent spread of wildtype HBV but antiviral treatment lead to resolution. Surprisingly the typical acute hepatitis B was not observed in a single one of the 26 cases of assumed or proven transmission.",

keywords = "Anti-HBc, Anti-HBs, Escape mutation, HBV DNA, Occult HBV infection, Window phase",

author = "Gerlich, {Wolfram H.} and Wagner, {Franz F.} and Michael Chudy and Harritshoj, {Lene Holm} and Annette Lattermann and Sandra Wienzek and Dieter Glebe and Mona Saniewski and Sch{\"u}ttler, {Christian G.} and Wend, {Ulrike C.} and Willems, {Wulf R.} and Ursula Bauerfeind and Christine Jork and Gregor Bein and Per Platz and Henrik Ullum and Ebbe Dickmeiss",

year = "2007",

month = oct,

day = "1",

doi = "10.1002/jmv.20963",

language = "English",

volume = "79",

journal = "Journal of Medical Virology",

issn = "0146-6615",

publisher = "JohnWiley & Sons, Inc.",

number = "SUPPL. 1",

}

TY - JOUR

T1 - HBsAg non-reactive HBV infection in blood donors

T2 - Transmission and pathogenicity

AU - Gerlich, Wolfram H.

AU - Wagner, Franz F.

AU - Chudy, Michael

AU - Harritshoj, Lene Holm

AU - Lattermann, Annette

AU - Wienzek, Sandra

AU - Glebe, Dieter

AU - Saniewski, Mona

AU - Schüttler, Christian G.

AU - Wend, Ulrike C.

AU - Willems, Wulf R.

AU - Bauerfeind, Ursula

AU - Jork, Christine

AU - Bein, Gregor

AU - Platz, Per

AU - Ullum, Henrik

AU - Dickmeiss, Ebbe

PY - 2007/10/1

Y1 - 2007/10/1

N2 - Five blood donors with occult persistent and one donor with an early window phase HBV infection were identified. They were negative in regular HBsAg screening and had low levels of HBV DNA that were probably not detectable by current mini-pool nucleic acid amplification testing. In four donors several mutations were found located in the HBs antigen loop. In three donors the mutations were predominantly outside the "a" determinant; one donor had a wild type HBsAg sequence. Fifty-five recipients of donations from the persistently infected donors could be tested for previous or ongoing HBV infection and of them 53% (29/55) were anti-HBc positive. Based on the prevalence of anti-HBc in Germany (7%), it was assumed that four of those recipients had already been positive before transfusion. In 22 cases, it was assumed that they acquired infection by the donations, but the infection remained asymptomatic and was resolved. In three cases transmission was proven by the time course of the acute infection and sequence identity The resulting infection was fatal and associated with immunological disorders at the time of transmission: in one case sepsis and in the other two cases immunosuppression. In a further asymptomatic case of proven transmission from the early window phase donation passively administered anti-HBs could not prevent spread of wildtype HBV but antiviral treatment lead to resolution. Surprisingly the typical acute hepatitis B was not observed in a single one of the 26 cases of assumed or proven transmission.

AB - Five blood donors with occult persistent and one donor with an early window phase HBV infection were identified. They were negative in regular HBsAg screening and had low levels of HBV DNA that were probably not detectable by current mini-pool nucleic acid amplification testing. In four donors several mutations were found located in the HBs antigen loop. In three donors the mutations were predominantly outside the "a" determinant; one donor had a wild type HBsAg sequence. Fifty-five recipients of donations from the persistently infected donors could be tested for previous or ongoing HBV infection and of them 53% (29/55) were anti-HBc positive. Based on the prevalence of anti-HBc in Germany (7%), it was assumed that four of those recipients had already been positive before transfusion. In 22 cases, it was assumed that they acquired infection by the donations, but the infection remained asymptomatic and was resolved. In three cases transmission was proven by the time course of the acute infection and sequence identity The resulting infection was fatal and associated with immunological disorders at the time of transmission: in one case sepsis and in the other two cases immunosuppression. In a further asymptomatic case of proven transmission from the early window phase donation passively administered anti-HBs could not prevent spread of wildtype HBV but antiviral treatment lead to resolution. Surprisingly the typical acute hepatitis B was not observed in a single one of the 26 cases of assumed or proven transmission.

KW - Anti-HBc

KW - Anti-HBs

KW - Escape mutation

KW - HBV DNA

KW - Occult HBV infection

KW - Window phase

UR - http://www.scopus.com/inward/record.url?scp=34748914837&partnerID=8YFLogxK

U2 - 10.1002/jmv.20963

DO - 10.1002/jmv.20963

M3 - Journal article

AN - SCOPUS:34748914837

SN - 0146-6615

VL - 79

JO - Journal of Medical Virology

JF - Journal of Medical Virology

IS - SUPPL. 1

ER -

HBsAg non-reactive HBV infection in blood donors: Transmission and pathogenicity

Abstract

FN’s Verdensmål

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater