Gene regulation in response to protein disulphide isomerase deficiency

Per Nørgaard; Christine Tachibana; Anette W Bruun; Jakob R. Winther

doi:10.1002/yea.978

Gene regulation in response to protein disulphide isomerase deficiency

Per Nørgaard, Christine Tachibana, Anette W Bruun, Jakob R. Winther

Biomolecular Sciences

5 Citationer (Scopus)

Abstract

We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.

Originalsprog	Engelsk
Tidsskrift	Yeast (Chichester, England)
Vol/bind	20
Udgave nummer	7
Sider (fra-til)	645-52
Antal sider	8
ISSN	0749-503X
DOI	https://doi.org/10.1002/yea.978
Status	Udgivet - 2003

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10.1002/yea.978

Citationsformater

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title = "Gene regulation in response to protein disulphide isomerase deficiency",

abstract = "We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.",

keywords = "Base Sequence, DNA, Fungal, Endoplasmic Reticulum, Gene Expression Regulation, Fungal, Genes, Fungal, Isomerases, Promoter Regions, Genetic, Protein Disulfide-Isomerases, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Deletion",

author = "Per N{\o}rgaard and Christine Tachibana and Bruun, {Anette W} and Winther, {Jakob R.}",

year = "2003",

doi = "10.1002/yea.978",

language = "English",

volume = "20",

pages = "645--52",

journal = "Yeast",

issn = "0749-503X",

publisher = "JohnWiley & Sons Ltd",

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TY - JOUR

T1 - Gene regulation in response to protein disulphide isomerase deficiency

AU - Nørgaard, Per

AU - Tachibana, Christine

AU - Bruun, Anette W

AU - Winther, Jakob R.

PY - 2003

Y1 - 2003

N2 - We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.

AB - We have examined the activities of promoters of a number of yeast genes encoding resident endoplasmic reticulum proteins, and found increased expression in a strain with severe protein disulphide isomerase deficiency. Serial deletion in the promoter of the MPD1 gene, which encodes a PDI1-homologue, revealed a cis-acting element responding to deficiency of protein disulphide isomerase activity (designated CERP). The presence of the sequence element is necessary and sufficient for the upregulation in response to disulphide isomerase deficiency, as measured by a minimal promoter containing the CERP element. The sequence (GACACG) does not resemble the unfolded protein response element. It is present in the upstream regions of the MPD1, MPD2, KAR2, PDI1 and ERO1 genes.

KW - Base Sequence

KW - DNA, Fungal

KW - Endoplasmic Reticulum

KW - Gene Expression Regulation, Fungal

KW - Genes, Fungal

KW - Isomerases

KW - Promoter Regions, Genetic

KW - Protein Disulfide-Isomerases

KW - Repressor Proteins

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Sequence Deletion

U2 - 10.1002/yea.978

DO - 10.1002/yea.978

M3 - Journal article

C2 - 12734802

SN - 0749-503X

VL - 20

SP - 645

EP - 652

JO - Yeast

JF - Yeast

IS - 7

ER -

Gene regulation in response to protein disulphide isomerase deficiency

Abstract

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