TY - JOUR
T1 - Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices
AU - Bakmand, Tanya
AU - Troels-Smith, Ane R.
AU - Dimaki, Maria
AU - Nissen, Jakob D.
AU - Andersen, Karsten B.
AU - Sasso, Luigi
AU - Waagepetersen, Helle S.
AU - Gramsbergen, Jan B.
AU - Svendsen, Winnie E.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.
AB - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.
KW - Fluidic
KW - Hippocampus
KW - Interface culturing
KW - Tissue culturing
UR - http://www.scopus.com/inward/record.url?scp=84933523234&partnerID=8YFLogxK
U2 - 10.1007/s10544-015-9973-6
DO - 10.1007/s10544-015-9973-6
M3 - Journal article
C2 - 25812519
AN - SCOPUS:84933523234
SN - 1387-2176
VL - 17
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 4
M1 - 71
ER -