Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen

P O Jensen; J Larsen; J K Larsen

Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen

P O Jensen, J Larsen, J K Larsen

Medical Genetics Program

8 Citationer (Scopus)

Abstract

RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.

Originalsprog	Engelsk
Tidsskrift	Acta Oncologica. Supplement
Vol/bind	32
Udgave nummer	5
Sider (fra-til)	521-4
Antal sider	4
ISSN	0284-186X
Status	Udgivet - 1993

Citationsformater

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title = "Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen",

abstract = "RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.",

keywords = "Antigens, CD, Antigens, CD13, Antigens, Differentiation, Myelomonocytic, Antigens, Neoplasm, Antigens, Surface, Cell Cycle, DNA, Neoplasm, Flow Cytometry, Humans, Leukemia, RNA, Neoplasm, Tumor Cells, Cultured, Uridine, Journal Article, Research Support, Non-U.S. Gov't",

author = "Jensen, {P O} and J Larsen and Larsen, {J K}",

year = "1993",

language = "English",

volume = "32",

pages = "521--4",

journal = "Acta Oncologica, Supplement",

issn = "1100-1704",

publisher = "Taylor & Francis",

number = "5",

}

TY - JOUR

T1 - Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen

AU - Jensen, P O

AU - Larsen, J

AU - Larsen, J K

PY - 1993

Y1 - 1993

N2 - RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.

AB - RNA synthesis can be analysed in nuclei or cells labelled with 5-bromouridine (BrUrd) and stained using cross-reacting anti-bromodeoxyuridine (BrdUrd) antibody. Flow cytometric dual parameter analysis of BrUrd incorporation and DNA content in nuclear suspensions of human blood lymphocytes showed that RNA synthesis increased within the first 24 hours of phytohemagglutinin (PHA) stimulation, reaching a maximum at 48 hours, when cells had entered the cell cycle. Using a new method for flow cytometric dual parameter analysis of BrUrd incorporation and a cell surface antigen, spontaneous RNA synthesis in HL-60 and K-562 cells was measured simultaneous with CD13 expression.

KW - Antigens, CD

KW - Antigens, CD13

KW - Antigens, Differentiation, Myelomonocytic

KW - Antigens, Neoplasm

KW - Antigens, Surface

KW - Cell Cycle

KW - DNA, Neoplasm

KW - Flow Cytometry

KW - Humans

KW - Leukemia

KW - RNA, Neoplasm

KW - Tumor Cells, Cultured

KW - Uridine

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 8105832

SN - 1100-1704

VL - 32

SP - 521

EP - 524

JO - Acta Oncologica, Supplement

JF - Acta Oncologica, Supplement

IS - 5

ER -

Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen

Abstract

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