Flow cytometric detection of wild yeast in lager breweries

TY - JOUR

T1 - Flow cytometric detection of wild yeast in lager breweries

AU - Jespersen, Lene

AU - Lassen, S-

AU - Jakobsen, Mogens

PY - 1993

Y1 - 1993

N2 - A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.

AB - A flow cytometric method for detection of wild yeast infections in breweries is reported. It is based on selective enrichment in Malt extract Yeast extract Glucose Peptone broth (MYGP) at 37 degrees C and in MYGP with 200 ppm CuSO4 at 25 degrees C, staining with a fluorochrome precursor and flow cytometry. In experiments with several types of wild yeast isolated from breweries and two different strains of lager yeast it has been possible to detect one wild yeast per 10(6) culture yeast after 48-72 h of incubation and, in some cases, after 24 h.

KW - Beer

KW - Evaluation Studies as Topic

KW - Flow Cytometry

KW - Food Microbiology

KW - Mycology

KW - Pichia

KW - Saccharomyces cerevisiae

U2 - 10.1016/0168-1605(93)90202-R

DO - 10.1016/0168-1605(93)90202-R

M3 - Journal article

C2 - 8466805

SN - 0168-1605

VL - 17

SP - 321

EP - 328

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - 4

ER -