Expression pattern of four membrane-type matrix metalloproteinases in the normal and diseased mouse mammary gland

TY - JOUR

T1 - Expression pattern of four membrane-type matrix metalloproteinases in the normal and diseased mouse mammary gland

AU - Szabova, Ludmila

AU - Yamada, Susan S

AU - Birkedal-Hansen, Henning

AU - Holmbeck, Kenn

PY - 2005/10

Y1 - 2005/10

N2 - Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse mammary gland sections. MT1-MMP and MT3-MMP were found in the mammary stroma mainly around epithelial structures in both developing and mature mammary gland. In contrast, MT2-MMP was found exclusively in the mammary epithelium. Lactating gland expressed none of the examined MT-MMPs. Mammary gland tumors expressed MT1-MMP, MT2-MMP, and MT3-MMP while MT4-MMP was not expressed in any developmental or cancerous stage analyzed here. Our results suggest that MT1-MMP, MT2-MMP, and MT3-MMP may be involved in remodeling of both the normal and diseased mammary gland either directly or indirectly by activation of other MMPs.

AB - Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse mammary gland sections. MT1-MMP and MT3-MMP were found in the mammary stroma mainly around epithelial structures in both developing and mature mammary gland. In contrast, MT2-MMP was found exclusively in the mammary epithelium. Lactating gland expressed none of the examined MT-MMPs. Mammary gland tumors expressed MT1-MMP, MT2-MMP, and MT3-MMP while MT4-MMP was not expressed in any developmental or cancerous stage analyzed here. Our results suggest that MT1-MMP, MT2-MMP, and MT3-MMP may be involved in remodeling of both the normal and diseased mammary gland either directly or indirectly by activation of other MMPs.

KW - Aging/physiology

KW - Animals

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Enzymologic/genetics

KW - Health

KW - Lactation/genetics

KW - Mammary Glands, Animal/cytology

KW - Mammary Neoplasms, Animal/enzymology

KW - Matrix Metalloproteinase 14

KW - Matrix Metalloproteinase 15

KW - Matrix Metalloproteinase 16

KW - Matrix Metalloproteinases, Membrane-Associated

KW - Metalloendopeptidases/chemistry

KW - Mice

KW - Pregnancy

U2 - 10.1002/jcp.20385

DO - 10.1002/jcp.20385

M3 - Journal article

C2 - 15895410

SN - 0021-9541

VL - 205

SP - 123

EP - 132

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 1

ER -