Expression of the epidermal growth factor receptor in human small cell lung cancer cell lines

TY - JOUR

T1 - Expression of the epidermal growth factor receptor in human small cell lung cancer cell lines

AU - Damstrup, L

AU - Rygaard, K

AU - Spang-Thomsen, M

AU - Poulsen, H S

N1 - Keywords: Blotting, Northern; Carcinoma, Small Cell; Cell Line; Epidermal Growth Factor; Humans; Kinetics; Lung Neoplasms; Molecular Weight; RNA, Messenger; Radioligand Assay; Receptor, Epidermal Growth Factor; Transforming Growth Factor alpha; Transforming Growth Factor beta

PY - 1992

Y1 - 1992

N2 - Epidermal growth factor (EGF) receptor expression was evaluated in a panel of 21 small cell lung cancer cell lines with radioreceptor assay, affinity labeling, and Northern blotting. We found high-affinity receptors to be expressed in 10 cell lines. Scatchard analysis of the binding data demonstrated that the cells bound between 3 and 52 fmol/mg protein with a KD ranging from 0.5 x 10(-10) to 2.7 x 10(-10) M. EGF binding to the receptor was confirmed by affinity-labeling EGF to the EGF receptor. The cross-linked complex had a M(r) of 170,000-180,000. Northern blotting showed the expression of EGF receptor mRNA in all 10 cell lines that were found to be EGF receptor-positive and in one cell line that was found to be EGF receptor-negative in the radioreceptor assay and affinity labeling. Our results provide, for the first time, evidence that a large proportion of a broad panel of small cell lung cancer cell lines express the EGF receptor.

AB - Epidermal growth factor (EGF) receptor expression was evaluated in a panel of 21 small cell lung cancer cell lines with radioreceptor assay, affinity labeling, and Northern blotting. We found high-affinity receptors to be expressed in 10 cell lines. Scatchard analysis of the binding data demonstrated that the cells bound between 3 and 52 fmol/mg protein with a KD ranging from 0.5 x 10(-10) to 2.7 x 10(-10) M. EGF binding to the receptor was confirmed by affinity-labeling EGF to the EGF receptor. The cross-linked complex had a M(r) of 170,000-180,000. Northern blotting showed the expression of EGF receptor mRNA in all 10 cell lines that were found to be EGF receptor-positive and in one cell line that was found to be EGF receptor-negative in the radioreceptor assay and affinity labeling. Our results provide, for the first time, evidence that a large proportion of a broad panel of small cell lung cancer cell lines express the EGF receptor.

M3 - Journal article

C2 - 1317257

SN - 0008-5472

VL - 52

SP - 3089

EP - 3093

JO - Cancer Research

JF - Cancer Research

IS - 11

ER -