Expression, content, and localization of insulin-like growth factor I in human achilles tendon

TY - JOUR

T1 - Expression, content, and localization of insulin-like growth factor I in human achilles tendon

AU - Olesen, Jens L

AU - Heinemeier, Katja M

AU - Langberg, Henning

AU - Magnusson, S Peter

AU - Kjaer, Michael

AU - Flyvbjerg, Allan

PY - 2006

Y1 - 2006

N2 - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.

AB - In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.

KW - Achilles Tendon

KW - Adult

KW - Fluorescent Antibody Technique, Indirect

KW - Gene Expression

KW - Humans

KW - Insulin-Like Growth Factor Binding Protein 4

KW - Insulin-Like Growth Factor I

KW - Male

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1080/03008200600809893

DO - 10.1080/03008200600809893

M3 - Journal article

C2 - 16987751

SN - 0300-8207

VL - 47

SP - 200

EP - 206

JO - Connective Tissue Research

JF - Connective Tissue Research

IS - 4

ER -