@article{70a050a0e7be11ddbf70000ea68e967b,
title = "Evidence for biosynthesis of lactase-phlorizin hydrolase as a single-chain high-molecular weight precursor",
abstract = "Precursor forms of lactase-phlorizin hydrolase, sucrase-isomaltase and aminopeptidase N were studied by pulse-labelling of organ-cultured human intestinal biopsies. After labelling the biopsies were fractionated by the Ca2+-precipitation method and the enzymes isolated by immunoprecipitation. The results indicate that the lactase-phlorizin hydrolase is synthesized as a Mr 245 000 polypeptide, which is intracellularly cleaved into its mature Mr 160 000 form. Sucrase-isomaltase is shown to be synthesized as a single chain precursor (Mr 245 000 and 265 000) while the precursor of aminopeptidase N is shown to be of apparently the same size as the mature enzyme (Mr 140 000 and 160 000).",
author = "H Skovbjerg and Danielsen, {E M} and Ove Noren and H Sj{\"o}str{\"o}m",
note = "Keywords: Aminopeptidases; Antigens, CD13; Electrophoresis, Polyacrylamide Gel; Enzyme Precursors; Glucosidases; Glycosylceramidase; Humans; Intestine, Small; Molecular Weight; Multienzyme Complexes; Organ Culture Techniques; Sucrase-Isomaltase Complex; beta-Galactosidase",
year = "1984",
language = "English",
volume = "798",
pages = "247--51",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",
}