@article{9aef8d30955a11df928f000ea68e967b,
title = "Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements",
abstract = "Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.",
author = "Alicia Lundby and Hiroki Mutoh and Dimitar Dimitrov and Walther Akemann and Thomas Kn{\"o}pfel",
note = "Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats",
year = "2008",
doi = "10.1371/journal.pone.0002514",
language = "English",
volume = "3",
pages = "e2514",
journal = "PLoS Computational Biology",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",
}