Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes

Patrick H Demana; Christian Fehske; Karen White; Thomas Rades; Sarah Hook

doi:10.1111/j.0818-9641.2004.01276.x

Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes

Patrick H Demana, Christian Fehske, Karen White, Thomas Rades, Sarah Hook

27 Citationer (Scopus)

Abstract

Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.

Originalsprog	Engelsk
Tidsskrift	Immunology and Cell Biology
Vol/bind	82
Udgave nummer	5
Sider (fra-til)	547-54
Antal sider	8
ISSN	0818-9641
DOI	https://doi.org/10.1111/j.0818-9641.2004.01276.x
Status	Udgivet - okt. 2004
Udgivet eksternt	Ja

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10.1111/j.0818-9641.2004.01276.x

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title = "Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes",

abstract = "Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.",

keywords = "Adjuvants, Immunologic, Animals, Antigens, Bone Marrow Cells, Dendritic Cells, Drug Delivery Systems, Liposomes, Lymphocyte Activation, Mice, Mice, Inbred Strains, Particle Size, Saponins, T-Lymphocytes",

author = "Demana, {Patrick H} and Christian Fehske and Karen White and Thomas Rades and Sarah Hook",

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doi = "10.1111/j.0818-9641.2004.01276.x",

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T1 - Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes

AU - Demana, Patrick H

AU - Fehske, Christian

AU - White, Karen

AU - Rades, Thomas

AU - Hook, Sarah

PY - 2004/10

Y1 - 2004/10

N2 - Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.

AB - Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.

KW - Adjuvants, Immunologic

KW - Animals

KW - Antigens

KW - Bone Marrow Cells

KW - Dendritic Cells

KW - Drug Delivery Systems

KW - Liposomes

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred Strains

KW - Particle Size

KW - Saponins

KW - T-Lymphocytes

U2 - 10.1111/j.0818-9641.2004.01276.x

DO - 10.1111/j.0818-9641.2004.01276.x

M3 - Journal article

C2 - 15479441

SN - 0818-9641

VL - 82

SP - 547

EP - 554

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

IS - 5

ER -

Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes

Abstract

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