Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins

TY - JOUR

T1 - Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins

AU - Moheimani, Fatemeh

AU - Tan, Joanne T M

AU - Brown, Bronwyn E

AU - Heather, Alison K

AU - van Reyk, David M

AU - Davies, Michael Jonathan

PY - 2011

Y1 - 2011

N2 - During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. Here we examined the hypothesis that hyperglycaemia may modulate receptor expression and hence lipid accumulation in macrophages. Human monocytes were matured into macrophages in 30 versus 5 mM glucose and receptor expression and lipid accumulation quantified. High glucose elevated LOX1 mRNA, but decreased SR-AI, SR-BI, LDLR, and CD36 mRNA. SR-BI and CD36 protein levels were decreased. Normo- and hyperglycaemic cells accumulated cholesteryl esters from modified LDL to a greater extent than control LDL, but total and individual cholesteryl ester accumulation was not affected by glucose levels. It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined.

AB - During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. Here we examined the hypothesis that hyperglycaemia may modulate receptor expression and hence lipid accumulation in macrophages. Human monocytes were matured into macrophages in 30 versus 5 mM glucose and receptor expression and lipid accumulation quantified. High glucose elevated LOX1 mRNA, but decreased SR-AI, SR-BI, LDLR, and CD36 mRNA. SR-BI and CD36 protein levels were decreased. Normo- and hyperglycaemic cells accumulated cholesteryl esters from modified LDL to a greater extent than control LDL, but total and individual cholesteryl ester accumulation was not affected by glucose levels. It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined.

KW - Antigens, CD36

KW - Atherosclerosis

KW - Base Sequence

KW - Cell Differentiation

KW - Cells, Cultured

KW - Cholesterol Esters

KW - DNA Primers

KW - Glucose

KW - Humans

KW - Lipid Metabolism

KW - Lipoproteins, LDL

KW - Macrophages

KW - RNA, Messenger

KW - Receptors, LDL

KW - Scavenger Receptors, Class A

KW - Scavenger Receptors, Class B

KW - Scavenger Receptors, Class E

U2 - 10.1155/2011/851280

DO - 10.1155/2011/851280

M3 - Journal article

C2 - 21904540

SN - 2314-6745

VL - 2011

SP - 851280

JO - Journal of Diabetes Research

JF - Journal of Diabetes Research

ER -