Early complement activation follows eversion carotid endarterectomy and correlates with the time of clamping of the carotid artery

TY - JOUR

T1 - Early complement activation follows eversion carotid endarterectomy and correlates with the time of clamping of the carotid artery

AU - Széplaki, Gábor

AU - Hirschberg, Kristóf

AU - Gombos, Tímea

AU - Varga, Lilian

AU - Prohászka, Zoltán

AU - Dósa, Edit

AU - Acsády, György

AU - Karádi, István

AU - Garred, Peter

AU - Entz, László

AU - Füst, George

N1 - Keywords: Adult; Aged; Aged, 80 and over; C-Reactive Protein; Carotid Arteries; Complement Activation; Complement C3a; Constriction; Endarterectomy, Carotid; Female; Genotype; Humans; Male; Mannose-Binding Lectin; Middle Aged; Reperfusion Injury; Stents; Time Factors

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Complement activation plays an important role in ischemia/reperfusion (I/R) injury. The objective of the present study was to detect the presence and mechanism of complement activation in patients who underwent carotid endarterectomy (CEA). METHODS: Complement activation products C1rsC1-inhibitor, C4d, C3a and SC5b-9 and concentrations of C-reactive protein (CRP) were measured in samples serially taken from 16 patients with eversion CEA and 10 with carotid artery stenting (CAS) in the first 24h post-surgery/intervention. MBL2 genotypes were also determined. RESULTS: In patients with CEA an intense increase in C3a levels were observed immediately after surgery (p<0.001), accompanied by a slight elevation in SC5b-9 levels (p<0.05). C3a levels remained elevated until 4h post-surgery, compared with the baseline values and with CAS patients. Peak C3a levels correlated with the time of carotid clamping (r=0.5921, p=0.02). No significant changes were detected in C1rsC1-inhibitor or C4d levels following CEA, and we found no association between the generation of C3a and MBL2 genotypes or CRP levels. Complement activation was not present in patients with CAS. CONCLUSIONS: Early complement activation follows CEA and correlates with the time of I/R injury. The lack of C4d generation suggests the role of the alternative and not the lectin pathway in the process Udgivelsesdato: 2008/6

AB - BACKGROUND: Complement activation plays an important role in ischemia/reperfusion (I/R) injury. The objective of the present study was to detect the presence and mechanism of complement activation in patients who underwent carotid endarterectomy (CEA). METHODS: Complement activation products C1rsC1-inhibitor, C4d, C3a and SC5b-9 and concentrations of C-reactive protein (CRP) were measured in samples serially taken from 16 patients with eversion CEA and 10 with carotid artery stenting (CAS) in the first 24h post-surgery/intervention. MBL2 genotypes were also determined. RESULTS: In patients with CEA an intense increase in C3a levels were observed immediately after surgery (p<0.001), accompanied by a slight elevation in SC5b-9 levels (p<0.05). C3a levels remained elevated until 4h post-surgery, compared with the baseline values and with CAS patients. Peak C3a levels correlated with the time of carotid clamping (r=0.5921, p=0.02). No significant changes were detected in C1rsC1-inhibitor or C4d levels following CEA, and we found no association between the generation of C3a and MBL2 genotypes or CRP levels. Complement activation was not present in patients with CAS. CONCLUSIONS: Early complement activation follows CEA and correlates with the time of I/R injury. The lack of C4d generation suggests the role of the alternative and not the lectin pathway in the process Udgivelsesdato: 2008/6

U2 - 10.1016/j.molimm.2008.02.011

DO - 10.1016/j.molimm.2008.02.011

M3 - Journal article

C2 - 18374983

SN - 0161-5890

VL - 45

SP - 3289

EP - 3294

JO - Molecular Immunology

JF - Molecular Immunology

IS - 11

ER -