Dynamic complex formation during the yeast cell cycle

Ulrik de Lichtenberg; Lars Juhl Jensen; Søren Brunak; Peer Bork

doi:10.1126/science.1105103

Dynamic complex formation during the yeast cell cycle

Ulrik de Lichtenberg, Lars Juhl Jensen, Søren Brunak, Peer Bork

330 Citationer (Scopus)

Abstract

To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

Originalsprog	Engelsk
Tidsskrift	Science (New York, N.Y.)
Vol/bind	307
Udgave nummer	5710
Sider (fra-til)	724-7
Antal sider	4
DOI	https://doi.org/10.1126/science.1105103
Status	Udgivet - 2005
Udgivet eksternt	Ja

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10.1126/science.1105103

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abstract = "To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.",

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AU - de Lichtenberg, Ulrik

AU - Jensen, Lars Juhl

AU - Brunak, Søren

AU - Bork, Peer

PY - 2005

Y1 - 2005

N2 - To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

AB - To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

U2 - 10.1126/science.1105103

DO - 10.1126/science.1105103

M3 - Journal article

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SN - 0036-8075

VL - 307

SP - 724

EP - 727

JO - Science

JF - Science

IS - 5710

ER -

Dynamic complex formation during the yeast cell cycle

Abstract

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