Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

Annegret  Wiedemann; Tamara Katharina Kakoschke; Cornelia Speth; Günter Rambach; Christian Ensinger; Henrik Elvang Jensen; Frank Ebel

doi:10.1016/j.ijmm.2016.05.002

Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

Annegret Wiedemann, Tamara Katharina Kakoschke, Cornelia Speth, Günter Rambach, Christian Ensinger, Henrik Elvang Jensen, Frank Ebel

7 Citationer (Scopus)

Abstract

Detection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. For diagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic structure of which consists of chains of several galactofuranose moieties. In this study, we provide evidence that Fusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannan and larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumigatus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions of hyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly suggests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 and a galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusarium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears to be a promising target antigen for a serological detection of Fusarium infections.

Originalsprog	Engelsk
Tidsskrift	International Journal of Medical Microbiology
Vol/bind	306
Sider (fra-til)	381-390
ISSN	1438-4221
DOI	https://doi.org/10.1016/j.ijmm.2016.05.002
Status	Udgivet - 1 sep. 2016

Adgang til dokumentet

10.1016/j.ijmm.2016.05.002

Citationsformater

Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret. / Wiedemann, Annegret ; Kakoschke, Tamara Katharina; Speth, Cornelia et al.

I: International Journal of Medical Microbiology, Bind 306, 01.09.2016, s. 381-390.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{c29286033a4b4b38ba3a2df3c0302147,

title = "Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret",

abstract = "Detection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. For diagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic structure of which consists of chains of several galactofuranose moieties. In this study, we provide evidence that Fusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannan and larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumigatus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions of hyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly suggests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 and a galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusarium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears to be a promising target antigen for a serological detection of Fusarium infections.",

author = "Annegret Wiedemann and Kakoschke, {Tamara Katharina} and Cornelia Speth and G{\"u}nter Rambach and Christian Ensinger and Jensen, {Henrik Elvang} and Frank Ebel",

year = "2016",

month = sep,

day = "1",

doi = "10.1016/j.ijmm.2016.05.002",

language = "English",

volume = "306",

pages = "381--390",

journal = "International Journal of Medical Microbiology",

issn = "1438-4221",

publisher = "Elsevier GmbH - Urban und Fischer",

}

TY - JOUR

T1 - Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

AU - Wiedemann, Annegret

AU - Kakoschke, Tamara Katharina

AU - Speth, Cornelia

AU - Rambach, Günter

AU - Ensinger, Christian

AU - Jensen, Henrik Elvang

AU - Ebel, Frank

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Detection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. For diagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic structure of which consists of chains of several galactofuranose moieties. In this study, we provide evidence that Fusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannan and larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumigatus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions of hyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly suggests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 and a galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusarium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears to be a promising target antigen for a serological detection of Fusarium infections.

AB - Detection of carbohydrate antigens is an important means for diagnosis of invasive fungal infections. For diagnosis of systemic Aspergillus infections, galactomannan is commonly used, the core antigenic structure of which consists of chains of several galactofuranose moieties. In this study, we provide evidence that Fusarium produces at least two distinct galactofuranose antigens: Smaller amounts of galactomannan and larger quantities of a novel antigen recognized by the monoclonal antibody AB135-8. In A. fumigatus, only minor amounts of the AB135-8 antigen are found in supernatants and in the apical regions of hyphae. A galactofuranose-deficient A. fumigatus mutant lacks the AB135-8 antigen, which strongly suggests that galactofuranose is an essential constituent of this antigen. Using a combination of AB135-8 and a galactomannan-specific antibody, we were able to unambiguously differentiate A. fumigatus and Fusarium hyphae in immunohistology. Moreover, since Fusarium releases the AB135-8 antigen, it appears to be a promising target antigen for a serological detection of Fusarium infections.

U2 - 10.1016/j.ijmm.2016.05.002

DO - 10.1016/j.ijmm.2016.05.002

M3 - Journal article

C2 - 27237422

SN - 1438-4221

VL - 306

SP - 381

EP - 390

JO - International Journal of Medical Microbiology

JF - International Journal of Medical Microbiology

ER -

Distinct galactofuranose antigens in the cell wall and culture supernatants as a means to differentiate Fusarium from AspergillusspeciesAnnegret

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater