Development of mammary glands of fat sheep submitted to restricted feeding during late pregnancy

Jan Værum Nørgaard; Mette Olaf Nielsen; Peter Kappel Theil; M.T. Sørensen; Sina Safayi; Kristen Sejrsen

doi:10.1016/j.smallrumres.2007.11.001

Development of mammary glands of fat sheep submitted to restricted feeding during late pregnancy

Jan Værum Nørgaard, Mette Olaf Nielsen, Peter Kappel Theil, M.T. Sørensen, Sina Safayi, Kristen Sejrsen

20 Citationer (Scopus)

Abstract

Mammary gland development in sheep occurs mainly during puberty and pregnancy. We have investigated the effects of a late gestation feed restriction on mammary gland development in sheep. Five control ewes were slaughtered d -38 from parturition, whereas 10 ewes were fed ad libitum and another 10 ewes were fed restrictedly (50% of energy and protein requirements) the last 38 days of pregnancy. Half the ewes in each feeding treatment group were slaughtered d -6 from parturition. Both mammary glands were collected at slaughter. From the remaining five sheep in each feeding group, blood and mammary biopsies were collected on d -16, d - 6, d 5 and d 30 from parturition. Fetus weights, weight of mammary parenchyma, amount of mammary epithelium and rate of mammary cell proliferation increased from d -38 to d -6 from parturition, but was not significantly affected by late gestation feed restriction. Colostrum yield was reduced (P=0.02) by feed restriction, but milk yield at d 5 and d 30 was not (P=0.12). Mammary gene expressions of alpha-lactalbumin (P=0.97), leptin (P=0.36), Bcl-2 (P=0.35), Bax (P=0.21), insulin-like growth factor I (IGF-I) (P=0.81) and cyclin D1 (P=0.43) were not affected by feed restriction, although plasma IGF-I (P=0.13) and leptin (P=0.04) concentrations were reduced by feed restriction. Our results suggest that extensive mobilization of body tissues occurred during late gestation feed restriction, which compensated for the reduced dietary nutrient supply, thereby maintaining fetal and mammary gland development, and this was likely explained by a high initial level of body fat in the experimental ewes, The colostrum yield was impaired by feed restrction, but our results show that in multiparous sheep, adequate post-partum nutrition can support lactational performance. In conclusion, mammary redevelopment in between successive lactations is not significantly affected by feed restriction during late pregnancy in fat sheep.

Originalsprog	Dansk
Tidsskrift	Small Ruminant Research
Vol/bind	76
Udgave nummer	3
Sider (fra-til)	155-165
Antal sider	11
ISSN	0921-4488
DOI	https://doi.org/10.1016/j.smallrumres.2007.11.001
Status	Udgivet - 2008

Emneord

Det tidligere LIFE

Adgang til dokumentet

10.1016/j.smallrumres.2007.11.001

Citationsformater

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title = "Development of mammary glands of fat sheep submitted to restricted feeding during late pregnancy",

abstract = "Mammary gland development in sheep occurs mainly during puberty and pregnancy. We have investigated the effects of a late gestation feed restriction on mammary gland development in sheep. Five control ewes were slaughtered d -38 from parturition, whereas 10 ewes were fed ad libitum and another 10 ewes were fed restrictedly (50% of energy and protein requirements) the last 38 days of pregnancy. Half the ewes in each feeding treatment group were slaughtered d -6 from parturition. Both mammary glands were collected at slaughter. From the remaining five sheep in each feeding group, blood and mammary biopsies were collected on d -16, d - 6, d 5 and d 30 from parturition. Fetus weights, weight of mammary parenchyma, amount of mammary epithelium and rate of mammary cell proliferation increased from d -38 to d -6 from parturition, but was not significantly affected by late gestation feed restriction. Colostrum yield was reduced (P=0.02) by feed restriction, but milk yield at d 5 and d 30 was not (P=0.12). Mammary gene expressions of alpha-lactalbumin (P=0.97), leptin (P=0.36), Bcl-2 (P=0.35), Bax (P=0.21), insulin-like growth factor I (IGF-I) (P=0.81) and cyclin D1 (P=0.43) were not affected by feed restriction, although plasma IGF-I (P=0.13) and leptin (P=0.04) concentrations were reduced by feed restriction. Our results suggest that extensive mobilization of body tissues occurred during late gestation feed restriction, which compensated for the reduced dietary nutrient supply, thereby maintaining fetal and mammary gland development, and this was likely explained by a high initial level of body fat in the experimental ewes, The colostrum yield was impaired by feed restrction, but our results show that in multiparous sheep, adequate post-partum nutrition can support lactational performance. In conclusion, mammary redevelopment in between successive lactations is not significantly affected by feed restriction during late pregnancy in fat sheep.",

keywords = "Det tidligere LIFE, Sheep, Mammary gland, Feed restriction, Pregnancy, Cell turnover",

author = "N{\o}rgaard, {Jan V{\ae}rum} and Nielsen, {Mette Olaf} and Theil, {Peter Kappel} and M.T. S{\o}rensen and Sina Safayi and Kristen Sejrsen",

year = "2008",

doi = "10.1016/j.smallrumres.2007.11.001",

language = "Dansk",

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pages = "155--165",

journal = "Small Ruminant Research",

issn = "0921-4488",

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TY - JOUR

T1 - Development of mammary glands of fat sheep submitted to restricted feeding during late pregnancy

AU - Nørgaard, Jan Værum

AU - Nielsen, Mette Olaf

AU - Theil, Peter Kappel

AU - Sørensen, M.T.

AU - Safayi, Sina

AU - Sejrsen, Kristen

PY - 2008

Y1 - 2008

N2 - Mammary gland development in sheep occurs mainly during puberty and pregnancy. We have investigated the effects of a late gestation feed restriction on mammary gland development in sheep. Five control ewes were slaughtered d -38 from parturition, whereas 10 ewes were fed ad libitum and another 10 ewes were fed restrictedly (50% of energy and protein requirements) the last 38 days of pregnancy. Half the ewes in each feeding treatment group were slaughtered d -6 from parturition. Both mammary glands were collected at slaughter. From the remaining five sheep in each feeding group, blood and mammary biopsies were collected on d -16, d - 6, d 5 and d 30 from parturition. Fetus weights, weight of mammary parenchyma, amount of mammary epithelium and rate of mammary cell proliferation increased from d -38 to d -6 from parturition, but was not significantly affected by late gestation feed restriction. Colostrum yield was reduced (P=0.02) by feed restriction, but milk yield at d 5 and d 30 was not (P=0.12). Mammary gene expressions of alpha-lactalbumin (P=0.97), leptin (P=0.36), Bcl-2 (P=0.35), Bax (P=0.21), insulin-like growth factor I (IGF-I) (P=0.81) and cyclin D1 (P=0.43) were not affected by feed restriction, although plasma IGF-I (P=0.13) and leptin (P=0.04) concentrations were reduced by feed restriction. Our results suggest that extensive mobilization of body tissues occurred during late gestation feed restriction, which compensated for the reduced dietary nutrient supply, thereby maintaining fetal and mammary gland development, and this was likely explained by a high initial level of body fat in the experimental ewes, The colostrum yield was impaired by feed restrction, but our results show that in multiparous sheep, adequate post-partum nutrition can support lactational performance. In conclusion, mammary redevelopment in between successive lactations is not significantly affected by feed restriction during late pregnancy in fat sheep.

AB - Mammary gland development in sheep occurs mainly during puberty and pregnancy. We have investigated the effects of a late gestation feed restriction on mammary gland development in sheep. Five control ewes were slaughtered d -38 from parturition, whereas 10 ewes were fed ad libitum and another 10 ewes were fed restrictedly (50% of energy and protein requirements) the last 38 days of pregnancy. Half the ewes in each feeding treatment group were slaughtered d -6 from parturition. Both mammary glands were collected at slaughter. From the remaining five sheep in each feeding group, blood and mammary biopsies were collected on d -16, d - 6, d 5 and d 30 from parturition. Fetus weights, weight of mammary parenchyma, amount of mammary epithelium and rate of mammary cell proliferation increased from d -38 to d -6 from parturition, but was not significantly affected by late gestation feed restriction. Colostrum yield was reduced (P=0.02) by feed restriction, but milk yield at d 5 and d 30 was not (P=0.12). Mammary gene expressions of alpha-lactalbumin (P=0.97), leptin (P=0.36), Bcl-2 (P=0.35), Bax (P=0.21), insulin-like growth factor I (IGF-I) (P=0.81) and cyclin D1 (P=0.43) were not affected by feed restriction, although plasma IGF-I (P=0.13) and leptin (P=0.04) concentrations were reduced by feed restriction. Our results suggest that extensive mobilization of body tissues occurred during late gestation feed restriction, which compensated for the reduced dietary nutrient supply, thereby maintaining fetal and mammary gland development, and this was likely explained by a high initial level of body fat in the experimental ewes, The colostrum yield was impaired by feed restrction, but our results show that in multiparous sheep, adequate post-partum nutrition can support lactational performance. In conclusion, mammary redevelopment in between successive lactations is not significantly affected by feed restriction during late pregnancy in fat sheep.

KW - Det tidligere LIFE

KW - Sheep

KW - Mammary gland

KW - Feed restriction

KW - Pregnancy

KW - Cell turnover

U2 - 10.1016/j.smallrumres.2007.11.001

DO - 10.1016/j.smallrumres.2007.11.001

M3 - Tidsskriftartikel

SN - 0921-4488

VL - 76

SP - 155

EP - 165

JO - Small Ruminant Research

JF - Small Ruminant Research

IS - 3

ER -