Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans

Lars Holm; Bruce O'Rourke; David Ebenstein; Michael J Toth; Rasmus Bechshoeft; Niels-Henrik Holstein-Rathlou; Michael Kjær; Dwight E Matthews

doi:10.1152/ajpendo.00579.2012

Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans

Lars Holm, Bruce O'Rourke, David Ebenstein, Michael J Toth, Rasmus Bechshoeft, Niels-Henrik Holstein-Rathlou, Michael Kjær, Dwight E Matthews

23 Citationer (Scopus)

Abstract

A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.

Originalsprog	Engelsk
Tidsskrift	A J P: Endocrinology and Metabolism (Online)
Vol/bind	304
Udgave nummer	8
Sider (fra-til)	E895-E907
Antal sider	13
ISSN	1522-1555
DOI	https://doi.org/10.1152/ajpendo.00579.2012
Status	Udgivet - 15 apr. 2013

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10.1152/ajpendo.00579.2012

Citationsformater

Holm, L., O'Rourke, B., Ebenstein, D., Toth, M. J., Bechshoeft, R., Holstein-Rathlou, N-H., Kjær, M., & Matthews, D. E. (2013). Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. A J P: Endocrinology and Metabolism (Online), 304(8), E895-E907. https://doi.org/10.1152/ajpendo.00579.2012

Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids : a method applicable in humans. / Holm, Lars; O'Rourke, Bruce; Ebenstein, David et al.

I: A J P: Endocrinology and Metabolism (Online), Bind 304, Nr. 8, 15.04.2013, s. E895-E907.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Holm, L, O'Rourke, B, Ebenstein, D, Toth, MJ, Bechshoeft, R, Holstein-Rathlou, N-H, Kjær, M & Matthews, DE 2013, 'Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans', A J P: Endocrinology and Metabolism (Online), bind 304, nr. 8, s. E895-E907. https://doi.org/10.1152/ajpendo.00579.2012

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title = "Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans",

abstract = "A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest. ",

author = "Lars Holm and Bruce O'Rourke and David Ebenstein and Toth, {Michael J} and Rasmus Bechshoeft and Niels-Henrik Holstein-Rathlou and Michael Kj{\ae}r and Matthews, {Dwight E}",

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T2 - a method applicable in humans

AU - Holm, Lars

AU - O'Rourke, Bruce

AU - Ebenstein, David

AU - Toth, Michael J

AU - Bechshoeft, Rasmus

AU - Holstein-Rathlou, Niels-Henrik

AU - Kjær, Michael

AU - Matthews, Dwight E

PY - 2013/4/15

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N2 - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.

AB - A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.

U2 - 10.1152/ajpendo.00579.2012

DO - 10.1152/ajpendo.00579.2012

M3 - Journal article

SN - 1522-1555

VL - 304

SP - E895-E907

JO - A J P: Endocrinology and Metabolism (Online)

JF - A J P: Endocrinology and Metabolism (Online)

IS - 8

ER -

Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans

Abstract

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