Deoxynivalenol transport across the human placental barrier

Jeanette K S Nielsen; Anna C Vikström; Paul Turner; Lisbeth E Knudsen

doi:10.1016/j.fct.2011.05.016

Deoxynivalenol transport across the human placental barrier

Jeanette K S Nielsen, Anna C Vikström, Paul Turner, Lisbeth E Knudsen

32 Citationer (Scopus)

Abstract

Deoxynivalenol (DON) is the most commonly detected mycotoxin contaminant of cereal crops and cereal based food products in temperate regions of the world. DON causes adverse health effects in animals, passes through to the foetus and causes foetal abnormalities in animals. Biomonitoring for DON has revealed frequent human exposure. This study reports on DON transfer across the human placenta. Firstly, in vitro studies with the BeWo b30 clone were used as a rapid screening model showing transfer of DON through a stable confluent cell monolayer. Five term placentas were then used to study DON transfer with the ex vivo dual perfusion model. The concentration of DON on the foetal side after 4h was about 21% of that on the maternal side at t=0. These results support the data from the BeWo monolayer model in respect to the transport rate of DON, and are consistent with our hypothesis of foetal exposure to DON during pregnancy.

Originalsprog	Engelsk
Tidsskrift	Food and Chemical Toxicology
Vol/bind	49
Udgave nummer	9
Sider (fra-til)	2046-52
Antal sider	7
ISSN	0278-6915
DOI	https://doi.org/10.1016/j.fct.2011.05.016
Status	Udgivet - sep. 2011

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10.1016/j.fct.2011.05.016

Citationsformater

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title = "Deoxynivalenol transport across the human placental barrier",

abstract = "Deoxynivalenol (DON) is the most commonly detected mycotoxin contaminant of cereal crops and cereal based food products in temperate regions of the world. DON causes adverse health effects in animals, passes through to the foetus and causes foetal abnormalities in animals. Biomonitoring for DON has revealed frequent human exposure. This study reports on DON transfer across the human placenta. Firstly, in vitro studies with the BeWo b30 clone were used as a rapid screening model showing transfer of DON through a stable confluent cell monolayer. Five term placentas were then used to study DON transfer with the ex vivo dual perfusion model. The concentration of DON on the foetal side after 4h was about 21% of that on the maternal side at t=0. These results support the data from the BeWo monolayer model in respect to the transport rate of DON, and are consistent with our hypothesis of foetal exposure to DON during pregnancy.",

keywords = "Cell Line, Enzyme-Linked Immunosorbent Assay, Female, Humans, Maternal-Fetal Exchange, Mycotoxins, Placenta, Pregnancy, Trichothecenes",

author = "Nielsen, {Jeanette K S} and Vikstr{\"o}m, {Anna C} and Paul Turner and Knudsen, {Lisbeth E}",

year = "2011",

month = sep,

doi = "10.1016/j.fct.2011.05.016",

language = "English",

volume = "49",

pages = "2046--52",

journal = "Food and Chemical Toxicology",

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TY - JOUR

T1 - Deoxynivalenol transport across the human placental barrier

AU - Nielsen, Jeanette K S

AU - Vikström, Anna C

AU - Turner, Paul

AU - Knudsen, Lisbeth E

PY - 2011/9

Y1 - 2011/9

N2 - Deoxynivalenol (DON) is the most commonly detected mycotoxin contaminant of cereal crops and cereal based food products in temperate regions of the world. DON causes adverse health effects in animals, passes through to the foetus and causes foetal abnormalities in animals. Biomonitoring for DON has revealed frequent human exposure. This study reports on DON transfer across the human placenta. Firstly, in vitro studies with the BeWo b30 clone were used as a rapid screening model showing transfer of DON through a stable confluent cell monolayer. Five term placentas were then used to study DON transfer with the ex vivo dual perfusion model. The concentration of DON on the foetal side after 4h was about 21% of that on the maternal side at t=0. These results support the data from the BeWo monolayer model in respect to the transport rate of DON, and are consistent with our hypothesis of foetal exposure to DON during pregnancy.

AB - Deoxynivalenol (DON) is the most commonly detected mycotoxin contaminant of cereal crops and cereal based food products in temperate regions of the world. DON causes adverse health effects in animals, passes through to the foetus and causes foetal abnormalities in animals. Biomonitoring for DON has revealed frequent human exposure. This study reports on DON transfer across the human placenta. Firstly, in vitro studies with the BeWo b30 clone were used as a rapid screening model showing transfer of DON through a stable confluent cell monolayer. Five term placentas were then used to study DON transfer with the ex vivo dual perfusion model. The concentration of DON on the foetal side after 4h was about 21% of that on the maternal side at t=0. These results support the data from the BeWo monolayer model in respect to the transport rate of DON, and are consistent with our hypothesis of foetal exposure to DON during pregnancy.

KW - Cell Line

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Maternal-Fetal Exchange

KW - Mycotoxins

KW - Placenta

KW - Pregnancy

KW - Trichothecenes

U2 - 10.1016/j.fct.2011.05.016

DO - 10.1016/j.fct.2011.05.016

M3 - Journal article

C2 - 21620924

SN - 0278-6915

VL - 49

SP - 2046

EP - 2052

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

IS - 9

ER -

Deoxynivalenol transport across the human placental barrier

Abstract

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