Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion

TY - JOUR

T1 - Delivery of endocytosed proteins to the cell-division plane requires change of pathway from recycling to secretion

AU - Richter, Sandra

AU - Kientz, Marika

AU - Brumm, Sabine

AU - Nielsen, Mads Eggert

AU - Park, Misoon

AU - Gavidia, Richard

AU - Krause, Cornelia

AU - Voss, Ute

AU - Beckmann, Hauke

AU - Mayer, Ulrike

AU - Stierhof, York Dieter

AU - Jürgens, Gerd

PY - 2014

Y1 - 2014

N2 - Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans- Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell-division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.

AB - Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans- Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell-division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint.

U2 - 10.7554/eLife.02131

DO - 10.7554/eLife.02131

M3 - Journal article

C2 - 24714496

AN - SCOPUS:84898471427

SN - 2050-084X

VL - 2014

JO - eLife

JF - eLife

IS - 3

M1 - e02131

ER -