Close-up view of the modifications of fluid membranes due to phospholipase A2

Ask F. Jakobsen; Ole G. Mouritsen; Matthias Weiss

doi:10.1088/0953-8984/17/47/025

Close-up view of the modifications of fluid membranes due to phospholipase A₂

Ask F. Jakobsen, Ole G. Mouritsen, Matthias Weiss^*

^*Corresponding author af dette arbejde

14 Citationer (Scopus)

Abstract

Phospholipases are a class of molecular machines that are involved in the active remodelling processes of biological membranes. These lipases are interfacially activated enzymes and in the specific case of phospholipase A ₂ (PLA₂) the enzyme catalyses the hydrolysis of di-acyl phospholipids into products of lysolipids and fatty acids, that dramatically change the physical properties of lipid membrane substrates. Using dissipative particle dynamics simulations on a simple coarse-grained bead-spring model of a fluid lipid bilayer in water, the mechanical and diffusive properties of the bilayer in the pure state and after the action of PLA₂ have been calculated. It is found that, in response to hydrolysis, the lipid membrane becomes mechanically softened and the various in-plane and trans-bilayer diffusional modes become enhanced. The results compare favourably with available experimental data.

Originalsprog	Engelsk
Tidsskrift	Journal of Physics Condensed Matter
Vol/bind	17
Udgave nummer	47
Sider (fra-til)	S4015-S4024
ISSN	0953-8984
DOI	https://doi.org/10.1088/0953-8984/17/47/025
Status	Udgivet - 30 nov. 2005

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10.1088/0953-8984/17/47/025

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Citationsformater

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title = "Close-up view of the modifications of fluid membranes due to phospholipase A2",

abstract = "Phospholipases are a class of molecular machines that are involved in the active remodelling processes of biological membranes. These lipases are interfacially activated enzymes and in the specific case of phospholipase A 2 (PLA2) the enzyme catalyses the hydrolysis of di-acyl phospholipids into products of lysolipids and fatty acids, that dramatically change the physical properties of lipid membrane substrates. Using dissipative particle dynamics simulations on a simple coarse-grained bead-spring model of a fluid lipid bilayer in water, the mechanical and diffusive properties of the bilayer in the pure state and after the action of PLA2 have been calculated. It is found that, in response to hydrolysis, the lipid membrane becomes mechanically softened and the various in-plane and trans-bilayer diffusional modes become enhanced. The results compare favourably with available experimental data.",

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T1 - Close-up view of the modifications of fluid membranes due to phospholipase A2

AU - Jakobsen, Ask F.

AU - Mouritsen, Ole G.

AU - Weiss, Matthias

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N2 - Phospholipases are a class of molecular machines that are involved in the active remodelling processes of biological membranes. These lipases are interfacially activated enzymes and in the specific case of phospholipase A 2 (PLA2) the enzyme catalyses the hydrolysis of di-acyl phospholipids into products of lysolipids and fatty acids, that dramatically change the physical properties of lipid membrane substrates. Using dissipative particle dynamics simulations on a simple coarse-grained bead-spring model of a fluid lipid bilayer in water, the mechanical and diffusive properties of the bilayer in the pure state and after the action of PLA2 have been calculated. It is found that, in response to hydrolysis, the lipid membrane becomes mechanically softened and the various in-plane and trans-bilayer diffusional modes become enhanced. The results compare favourably with available experimental data.

AB - Phospholipases are a class of molecular machines that are involved in the active remodelling processes of biological membranes. These lipases are interfacially activated enzymes and in the specific case of phospholipase A 2 (PLA2) the enzyme catalyses the hydrolysis of di-acyl phospholipids into products of lysolipids and fatty acids, that dramatically change the physical properties of lipid membrane substrates. Using dissipative particle dynamics simulations on a simple coarse-grained bead-spring model of a fluid lipid bilayer in water, the mechanical and diffusive properties of the bilayer in the pure state and after the action of PLA2 have been calculated. It is found that, in response to hydrolysis, the lipid membrane becomes mechanically softened and the various in-plane and trans-bilayer diffusional modes become enhanced. The results compare favourably with available experimental data.

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