Changes in protein expression in p53 deleted spontaneous thymic lymphomas.

TY - JOUR

T1 - Changes in protein expression in p53 deleted spontaneous thymic lymphomas.

AU - Honoré, Bent

AU - Vorum, Henrik

AU - Pedersen, Anders Elm

AU - Buus, Søren

AU - Claësson, Mogens H

N1 - Keywords: Animals; Bleomycin; Cell Line, Tumor; Drug Resistance, Multiple; Flow Cytometry; Gene Deletion; Gene Expression Regulation, Neoplastic; Genes, p53; Genetic Markers; Mice; Mice, Knockout; Neoplasm Proteins; Phosphoproteins; Ribosomal Proteins; T-Lymphocytes; Thymoma; Thymus Neoplasms; Tumor Suppressor Protein p53

PY - 2004

Y1 - 2004

N2 - By the use of high-resolution two-dimensional gel electrophoresis and computerized image analysis we investigated and compared the expression of cellular proteins from p53 positive (+/+) mouse thymocytes, p53-/- thymocytes before neoplastic transformation, and from cell lines derived from two spontaneous p53-/- thymic lymphomas, SM5 and SM7. A total of around 1500 proteins were detected on individual gels. Only changes in protein expression by a factor of 2 or more were considered. In the thymic lymphoma cells 3-5% of the proteins were found to be differentially regulated when compared with the protein expression in p53+/+ and p53-/- thymocytes. Only a minority (13 proteins) of the quantitatively changed proteins were common for the two thymic lymphoma cell lines, suggesting that the p53 deficiency mainly results in genetic dysfunctions which are individual for a given tumor. Two of the detected proteins increased their expression levels by more than 10 times from the p53+/+ to the p53-/- thymocytes and these high expression levels were also found in thymic lymphomas. The two proteins were identified by mass spectrometry as acidic ribosomal phosphoprotein P0 and a 33-kDa protein with a primary structure containing motifs of the glyoxalase-bleomycin resistance protein family (MDR) as deduced from the cDNA.

AB - By the use of high-resolution two-dimensional gel electrophoresis and computerized image analysis we investigated and compared the expression of cellular proteins from p53 positive (+/+) mouse thymocytes, p53-/- thymocytes before neoplastic transformation, and from cell lines derived from two spontaneous p53-/- thymic lymphomas, SM5 and SM7. A total of around 1500 proteins were detected on individual gels. Only changes in protein expression by a factor of 2 or more were considered. In the thymic lymphoma cells 3-5% of the proteins were found to be differentially regulated when compared with the protein expression in p53+/+ and p53-/- thymocytes. Only a minority (13 proteins) of the quantitatively changed proteins were common for the two thymic lymphoma cell lines, suggesting that the p53 deficiency mainly results in genetic dysfunctions which are individual for a given tumor. Two of the detected proteins increased their expression levels by more than 10 times from the p53+/+ to the p53-/- thymocytes and these high expression levels were also found in thymic lymphomas. The two proteins were identified by mass spectrometry as acidic ribosomal phosphoprotein P0 and a 33-kDa protein with a primary structure containing motifs of the glyoxalase-bleomycin resistance protein family (MDR) as deduced from the cDNA.

U2 - 10.1016/j.yexcr.2003.11.029

DO - 10.1016/j.yexcr.2003.11.029

M3 - Journal article

C2 - 15051493

SN - 0014-4827

VL - 295

SP - 91

EP - 101

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -