Cell wall composition and candidate biosynthesis gene expression during rice development

Fan Lin; Chithra Manisseri; Alexandra Fagerström; Matthew L. Peck; Miguel E. Vega-Sánchez; Brian Williams; Dawn M. Chiniquy; Prasenjit Saha; Sivakumar Pattathil; Brian Conlin; Lan Zhu; Michael G Hahn; William George Tycho Willats; Henrik V. Scheller; Pamela C. Ronald; Laura E. Bartley

doi:10.1093/pcp/pcw125

Cell wall composition and candidate biosynthesis gene expression during rice development

Fan Lin, Chithra Manisseri, Alexandra Fagerström, Matthew L. Peck, Miguel E. Vega-Sánchez, Brian Williams, Dawn M. Chiniquy, Prasenjit Saha, Sivakumar Pattathil, Brian Conlin, Lan Zhu, Michael G Hahn, William George Tycho Willats, Henrik V. Scheller, Pamela C. Ronald, Laura E. Bartley

Planteglycobiologi

10 Citationer (Scopus)

Abstract

Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.

Originalsprog	Engelsk
Tidsskrift	Plant and Cell Physiology
Vol/bind	57
Udgave nummer	10
Sider (fra-til)	2058-2075
Antal sider	18
ISSN	0032-0781
DOI	https://doi.org/10.1093/pcp/pcw125
Status	Udgivet - okt. 2016

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10.1093/pcp/pcw125

pcw125.pdfForlagets udgivne version, 1,76 MB

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Lin, F., Manisseri, C., Fagerström, A., Peck, M. L., Vega-Sánchez, M. E., Williams, B., Chiniquy, D. M., Saha, P., Pattathil, S., Conlin, B., Zhu, L., Hahn, M. G., Willats, W. G. T., Scheller, H. V., Ronald, P. C., & Bartley, L. E. (2016). Cell wall composition and candidate biosynthesis gene expression during rice development. Plant and Cell Physiology, 57(10), 2058-2075. https://doi.org/10.1093/pcp/pcw125

Lin, F, Manisseri, C, Fagerström, A, Peck, ML, Vega-Sánchez, ME, Williams, B, Chiniquy, DM, Saha, P, Pattathil, S, Conlin, B, Zhu, L, Hahn, MG, Willats, WGT, Scheller, HV, Ronald, PC & Bartley, LE 2016, 'Cell wall composition and candidate biosynthesis gene expression during rice development', Plant and Cell Physiology, bind 57, nr. 10, s. 2058-2075. https://doi.org/10.1093/pcp/pcw125

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title = "Cell wall composition and candidate biosynthesis gene expression during rice development",

abstract = "Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.",

author = "Fan Lin and Chithra Manisseri and Alexandra Fagerstr{\"o}m and Peck, {Matthew L.} and Vega-S{\'a}nchez, {Miguel E.} and Brian Williams and Chiniquy, {Dawn M.} and Prasenjit Saha and Sivakumar Pattathil and Brian Conlin and Lan Zhu and Hahn, {Michael G} and Willats, {William George Tycho} and Scheller, {Henrik V.} and Ronald, {Pamela C.} and Bartley, {Laura E.}",

note = "{\textcopyright} The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: [email protected].",

year = "2016",

month = oct,

doi = "10.1093/pcp/pcw125",

language = "English",

volume = "57",

pages = "2058--2075",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

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TY - JOUR

T1 - Cell wall composition and candidate biosynthesis gene expression during rice development

AU - Lin, Fan

AU - Manisseri, Chithra

AU - Fagerström, Alexandra

AU - Peck, Matthew L.

AU - Vega-Sánchez, Miguel E.

AU - Williams, Brian

AU - Chiniquy, Dawn M.

AU - Saha, Prasenjit

AU - Pattathil, Sivakumar

AU - Conlin, Brian

AU - Zhu, Lan

AU - Hahn, Michael G

AU - Willats, William George Tycho

AU - Scheller, Henrik V.

AU - Ronald, Pamela C.

AU - Bartley, Laura E.

PY - 2016/10

Y1 - 2016/10

N2 - Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.

AB - Cell walls of grasses, including cereal crops and biofuel grasses, comprise the majority of plant biomass and intimately influence plant growth, development and physiology. However, the functions of many cell wall synthesis genes, and the relationships among and the functions of cell wall components remain obscure. To better understand the patterns of cell wall accumulation and identify genes that act in grass cell wall biosynthesis, we characterized 30 samples from aerial organs of rice (Oryza sativa cv. Kitaake) at 10 developmental time points, 3-100 d post-germination. Within these samples, we measured 15 cell wall chemical components, enzymatic digestibility and 18 cell wall polysaccharide epitopes/ligands. We also used quantitative reverse transcription-PCR to measure expression of 50 glycosyltransferases, 15 acyltransferases and eight phenylpropanoid genes, many of which had previously been identified as being highly expressed in rice. Most cell wall components vary significantly during development, and correlations among them support current understanding of cell walls. We identified 92 significant correlations between cell wall components and gene expression and establish nine strong hypotheses for genes that synthesize xylans, mixed linkage glucan and pectin components. This work provides an extensive analysis of cell wall composition throughout rice development, identifies genes likely to synthesize grass cell walls, and provides a framework for development of genetically improved grasses for use in lignocellulosic biofuel production and agriculture.

U2 - 10.1093/pcp/pcw125

DO - 10.1093/pcp/pcw125

M3 - Journal article

C2 - 27481893

SN - 0032-0781

VL - 57

SP - 2058

EP - 2075

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 10

ER -

Cell wall composition and candidate biosynthesis gene expression during rice development

Abstract

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