Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles: Characterization, antimicrobial activity, and toxicity studies

TY - JOUR

T1 - Canna edulis leaf extract-mediated preparation of stabilized silver nanoparticles

T2 - Characterization, antimicrobial activity, and toxicity studies

AU - Otari, S. V.

AU - Pawar, S. H.

AU - Patel, Sanjay K.S.

AU - Singh, Raushan K.

AU - Kim, Sang Yong

AU - Lee, Jai Hyo

AU - Zhang, Liaoyuan

AU - Lee, Jung Kul

PY - 2017/4

Y1 - 2017/4

N2 - A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.

AB - A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.

KW - Antimicrobial

KW - Biocompatible

KW - Canna edulis Ker-Gawl

KW - Green chemistry

KW - Nanobiotechnology

UR - http://www.scopus.com/inward/record.url?scp=85018341752&partnerID=8YFLogxK

U2 - 10.4014/jmb.1610.10019

DO - 10.4014/jmb.1610.10019

M3 - Journal article

C2 - 28081356

AN - SCOPUS:85018341752

SN - 1598-642X

VL - 27

SP - 731

EP - 738

JO - Korean Journal of Applied Microbiology and Biotechnology

JF - Korean Journal of Applied Microbiology and Biotechnology

IS - 4

ER -