Ca2+ response in neutrophils after exposure to bacterial N-formyl-methionyl-leucyl-phenylalanine: delayed response in ulcerative colitis

TY - JOUR

T1 - Ca2+ response in neutrophils after exposure to bacterial N-formyl-methionyl-leucyl-phenylalanine: delayed response in ulcerative colitis

AU - Vainer, Ben

AU - Lamberth, Kasper

AU - Brimnes, Jens

AU - Nielsen, Ole H

AU - Claesson, Mogens Helweg

PY - 2003/3/1

Y1 - 2003/3/1

N2 - In acute stages of ulcerative colitis (UC), neutrophils migrate from the circulation into inflamed colonic tissue, initiated by yet unknown stimuli. The bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) is a component of the surface membrane of colonic bacteria such as Escherichia coli and stimulates Ca2+ influx into neutrophils, reflecting the fact that ionized calcium is an important secondary messenger for several neutrophil functions, including locomotion, phagocytosis and free oxygen radical production. Recent studies have revealed that Ca2+ dependent ICAM-1/beta 2-integrin mediated neutrophil migration is impaired in UC patients. The aim of the present work was to study the influx of Ca2+ into peripheral blood neutrophils of UC patients after exposure to FMLP and after binding of either beta 2-integrins or intercellular adhesion molecule-1 (ICAM-1).

AB - In acute stages of ulcerative colitis (UC), neutrophils migrate from the circulation into inflamed colonic tissue, initiated by yet unknown stimuli. The bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) is a component of the surface membrane of colonic bacteria such as Escherichia coli and stimulates Ca2+ influx into neutrophils, reflecting the fact that ionized calcium is an important secondary messenger for several neutrophil functions, including locomotion, phagocytosis and free oxygen radical production. Recent studies have revealed that Ca2+ dependent ICAM-1/beta 2-integrin mediated neutrophil migration is impaired in UC patients. The aim of the present work was to study the influx of Ca2+ into peripheral blood neutrophils of UC patients after exposure to FMLP and after binding of either beta 2-integrins or intercellular adhesion molecule-1 (ICAM-1).

KW - Adult

KW - Aged

KW - Antigens, CD11

KW - Calcium

KW - Cell Adhesion Molecules

KW - Cell Movement

KW - Cells, Cultured

KW - Colitis, Ulcerative

KW - Cytosol

KW - Humans

KW - Male

KW - Middle Aged

KW - N-Formylmethionine Leucyl-Phenylalanine

KW - Neutrophils

KW - Time Factors

KW - Up-Regulation

U2 - 10.1097/01.meg.0000050012.68425.59

DO - 10.1097/01.meg.0000050012.68425.59

M3 - Journal article

C2 - 12610322

SN - 0954-691X

VL - 15

SP - 267

EP - 273

JO - European Journal of Gastroenterology and Hepatology, Supplement

JF - European Journal of Gastroenterology and Hepatology, Supplement

IS - 3

ER -