Abstract
Cumulus-oocyte complexes were obtained from cows by aspiration of small (1-6 mm in diameter) antral follicles after slaughter. Complexes with a compact multilayered cumulus investment were cultured and processed for transmission electron microscopy after different periods of culture including a 0 h control group. In 0 h control oocytes the cumulus cells had numerous projections which penetrated the zona pellucida and established gap junctions with the oolemma. A partial loss of these junctions was noticed as an early event of oocyte maturation occurring within the first 3 h of culture. A low frequency of gap junctions was maintained until 12-18 h of culture where the junctional contact was completely disrupted. This decrease in intercellular communication was parallelled by resumption of oocyte meiosis.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Brain Structure and Function (Print Edition) |
| Vol/bind | 176 |
| Sider (fra-til) | 41-44 |
| Antal sider | 4 |
| ISSN | 1863-2653 |
| Status | Udgivet - 1987 |
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