Automatic inducer addition and harvesting of recombinant Escherichia coli cultures based on indirect on-line estimation of biomass concentration and specific growth rate

Teknik og materialevidenskab

• Biomass 49%
• Bioreactors 10%
• Cells 26%
• Cooling 6%
• Dissolved oxygen 11%
• Escherichia coli 68%
• Fusion reactions 7%
• Glucose 10%
• Glutathione 100%
• Ligands 12%
• Peroxisome Proliferator-Activated Receptors 18%
• Personnel 6%
• Proteins 41%
• Recombinant proteins 15%
• Sodium hydroxide 10%
• Substrates 6%

Medicin og biovidenskab

• Biomass 67%
• Bioreactors 9%
• Cell Division 22%
• Escherichia coli 38%
• Glucose 4%
• Glutathione Transferase 49%
• Growth 27%
• Growth Inhibitors 10%
• Inclusion Bodies 8%
• Ligands 5%
• Oxygen 5%
• Peroxisome Proliferator-Activated Receptors 9%
• PPAR alpha 49%
• Protein Binding 7%
• Proteins 11%
• Recombinant Proteins 7%
• Suspensions 6%

Kemiske forbindelser

• Bioreactors 8%
• Cooling 6%
• Dioxygen 3%
• Glucose 5%
• Glutathione 41%
• Growth Inhibitor 10%
• Inducer 63%
• Length 9%
• Metabolic 6%
• pH Value 6%
• Protein 19%
• Reaction Yield 9%
• Suspension 6%
• Tension 7%
• Time 2%