Apple, cherry, and blackcurrant increases nuclear factor kappa B activation in liver of transgenic mice

Trude R. Balstad; Ingvild Paur; Morten Poulsen; Jaroslaw Markowski; Krzysztof Kolodziejczyk; Lars Ove Dragsted; Mari C.W. Myhrstad; Rune Blomhoff

doi:10.1080/01635581003695749

Apple, cherry, and blackcurrant increases nuclear factor kappa B activation in liver of transgenic mice

Trude R. Balstad, Ingvild Paur, Morten Poulsen, Jaroslaw Markowski, Krzysztof Kolodziejczyk, Lars Ove Dragsted, Mari C.W. Myhrstad, Rune Blomhoff

6 Citationer (Scopus)

Abstract

Nuclear factor kappa B (NF-B) is essential in normal physiology, and several human disorders involve inappropriate regulation of NF-κB. Diets dominated by plant-based foods protect against chronic diseases, and several food derived compounds have been identified as promising NF-κB modulators. We investigated the effects of diets supplemented with apple, blackcurrant, or cherries on lipopolysaccharide (LPS)-induced NF-κB activation in transgenic NF-κB-luciferase mice. Whole body and organ specific NF-κB activities were determined. The mice had ad libitum access to the respective experimental diets for 7 days. On Day 7, all mice were given an LPS-injection (2.5 mg/kg), and NF-κB activation was monitored by in vivo imaging for 6 h. After imaging, blood samples were taken, the mice were euthanized, and ex vivo imaging of organs was performed. Compared to the control group, the apple and cherry groups had slightly higher whole-body NF-B activation at 4 h, and all 3 experimental groups had higher NF-κB activation at 6 h. LPS-induced NF-κB activation in liver was increased with all 3 experimental diets, but no effects were observed in other organs. Our findings indicate that high intakes of lyophilized fruits modulate in vivo NF-κB signaling in the liver following LPS-induced stress; however, consequences of this NF-κB modulation in hepatic tissue needs further investigation.

Originalsprog	Engelsk
Tidsskrift	Nutrition and Cancer
Vol/bind	62
Udgave nummer	6
Sider (fra-til)	841-848
Antal sider	8
ISSN	0163-5581
DOI	https://doi.org/10.1080/01635581003695749
Status	Udgivet - aug. 2010

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title = "Apple, cherry, and blackcurrant increases nuclear factor kappa B activation in liver of transgenic mice",

abstract = "Nuclear factor kappa B (NF-B) is essential in normal physiology, and several human disorders involve inappropriate regulation of NF-κB. Diets dominated by plant-based foods protect against chronic diseases, and several food derived compounds have been identified as promising NF-κB modulators. We investigated the effects of diets supplemented with apple, blackcurrant, or cherries on lipopolysaccharide (LPS)-induced NF-κB activation in transgenic NF-κB-luciferase mice. Whole body and organ specific NF-κB activities were determined. The mice had ad libitum access to the respective experimental diets for 7 days. On Day 7, all mice were given an LPS-injection (2.5 mg/kg), and NF-κB activation was monitored by in vivo imaging for 6 h. After imaging, blood samples were taken, the mice were euthanized, and ex vivo imaging of organs was performed. Compared to the control group, the apple and cherry groups had slightly higher whole-body NF-B activation at 4 h, and all 3 experimental groups had higher NF-κB activation at 6 h. LPS-induced NF-κB activation in liver was increased with all 3 experimental diets, but no effects were observed in other organs. Our findings indicate that high intakes of lyophilized fruits modulate in vivo NF-κB signaling in the liver following LPS-induced stress; however, consequences of this NF-κB modulation in hepatic tissue needs further investigation.",

author = "Balstad, {Trude R.} and Ingvild Paur and Morten Poulsen and Jaroslaw Markowski and Krzysztof Kolodziejczyk and Dragsted, {Lars Ove} and Myhrstad, {Mari C.W.} and Rune Blomhoff",

year = "2010",

month = aug,

doi = "10.1080/01635581003695749",

language = "English",

volume = "62",

pages = "841--848",

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TY - JOUR

T1 - Apple, cherry, and blackcurrant increases nuclear factor kappa B activation in liver of transgenic mice

AU - Balstad, Trude R.

AU - Paur, Ingvild

AU - Poulsen, Morten

AU - Markowski, Jaroslaw

AU - Kolodziejczyk, Krzysztof

AU - Dragsted, Lars Ove

AU - Myhrstad, Mari C.W.

AU - Blomhoff, Rune

PY - 2010/8

Y1 - 2010/8

N2 - Nuclear factor kappa B (NF-B) is essential in normal physiology, and several human disorders involve inappropriate regulation of NF-κB. Diets dominated by plant-based foods protect against chronic diseases, and several food derived compounds have been identified as promising NF-κB modulators. We investigated the effects of diets supplemented with apple, blackcurrant, or cherries on lipopolysaccharide (LPS)-induced NF-κB activation in transgenic NF-κB-luciferase mice. Whole body and organ specific NF-κB activities were determined. The mice had ad libitum access to the respective experimental diets for 7 days. On Day 7, all mice were given an LPS-injection (2.5 mg/kg), and NF-κB activation was monitored by in vivo imaging for 6 h. After imaging, blood samples were taken, the mice were euthanized, and ex vivo imaging of organs was performed. Compared to the control group, the apple and cherry groups had slightly higher whole-body NF-B activation at 4 h, and all 3 experimental groups had higher NF-κB activation at 6 h. LPS-induced NF-κB activation in liver was increased with all 3 experimental diets, but no effects were observed in other organs. Our findings indicate that high intakes of lyophilized fruits modulate in vivo NF-κB signaling in the liver following LPS-induced stress; however, consequences of this NF-κB modulation in hepatic tissue needs further investigation.

AB - Nuclear factor kappa B (NF-B) is essential in normal physiology, and several human disorders involve inappropriate regulation of NF-κB. Diets dominated by plant-based foods protect against chronic diseases, and several food derived compounds have been identified as promising NF-κB modulators. We investigated the effects of diets supplemented with apple, blackcurrant, or cherries on lipopolysaccharide (LPS)-induced NF-κB activation in transgenic NF-κB-luciferase mice. Whole body and organ specific NF-κB activities were determined. The mice had ad libitum access to the respective experimental diets for 7 days. On Day 7, all mice were given an LPS-injection (2.5 mg/kg), and NF-κB activation was monitored by in vivo imaging for 6 h. After imaging, blood samples were taken, the mice were euthanized, and ex vivo imaging of organs was performed. Compared to the control group, the apple and cherry groups had slightly higher whole-body NF-B activation at 4 h, and all 3 experimental groups had higher NF-κB activation at 6 h. LPS-induced NF-κB activation in liver was increased with all 3 experimental diets, but no effects were observed in other organs. Our findings indicate that high intakes of lyophilized fruits modulate in vivo NF-κB signaling in the liver following LPS-induced stress; however, consequences of this NF-κB modulation in hepatic tissue needs further investigation.

U2 - 10.1080/01635581003695749

DO - 10.1080/01635581003695749

M3 - Journal article

C2 - 20661833

SN - 0163-5581

VL - 62

SP - 841

EP - 848

JO - Nutrition and Cancer

JF - Nutrition and Cancer

IS - 6

ER -

Apple, cherry, and blackcurrant increases nuclear factor kappa B activation in liver of transgenic mice

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