AFLP analysis of genetic diversity in leafy kale (Brassica oleracea L. convar. acephala (DC.) Alef.) landraces, cultivars and wild populations in Europe

TY - JOUR

T1 - AFLP analysis of genetic diversity in leafy kale (Brassica oleracea L. convar. acephala (DC.) Alef.) landraces, cultivars and wild populations in Europe

AU - Christensen, Stina

AU - Bothmer, Roland von

AU - Poulsen, Gert

AU - Maggioni, Lorenzo

AU - Philip, Marianne

AU - Andersen, Bente Anni

AU - Bagger Jørgensen, Rikke

PY - 2011/6

Y1 - 2011/6

N2 - AFLP markers were used to characterize diversity and asses the genetic structure among 17 accessions of kale landraces, cultivars and wild populations from Europe. The range of average gene diversity in accessions was 0.11-0.27. Several landraces showed higher levels of diversity than the wild populations and one cultivar had the lowest diversity measures. The landraces that were most genetically diverse were from areas where kales are known to be extensively grown, suggesting in situ conservation in these areas as a supplement to storage of seeds in gene banks. An analysis of molecular variance (AMOVA) showed that 62% of the total variation was found within accessions. For most accessions, genetic distance was not related to geographic distance. Similarities among accessions were probably not caused by recent gene flow since they were widely separated geographically; more likely the relationship among them is due to seed dispersal through human interactions. Our results indicate that a kale population found in a natural habitat in Denmark was probably not truly wild but most likely an escape from a cultivated Danish kale that had subsequently become naturalized

AB - AFLP markers were used to characterize diversity and asses the genetic structure among 17 accessions of kale landraces, cultivars and wild populations from Europe. The range of average gene diversity in accessions was 0.11-0.27. Several landraces showed higher levels of diversity than the wild populations and one cultivar had the lowest diversity measures. The landraces that were most genetically diverse were from areas where kales are known to be extensively grown, suggesting in situ conservation in these areas as a supplement to storage of seeds in gene banks. An analysis of molecular variance (AMOVA) showed that 62% of the total variation was found within accessions. For most accessions, genetic distance was not related to geographic distance. Similarities among accessions were probably not caused by recent gene flow since they were widely separated geographically; more likely the relationship among them is due to seed dispersal through human interactions. Our results indicate that a kale population found in a natural habitat in Denmark was probably not truly wild but most likely an escape from a cultivated Danish kale that had subsequently become naturalized

KW - Brassica oleracea

KW - Genetic resources

KW - Molecular markers

KW - Genetic differentiation

KW - MULTILOCUS GENOTYPE DATA

KW - PLASMODIOPHORA-BRASSICAE

KW - CABBAGE

KW - MARKERS

KW - CONSERVATION

KW - INFERENCE

KW - ISOZYME

KW - FUTURE

KW - UK

U2 - 10.1007/s10722-010-9607-z

DO - 10.1007/s10722-010-9607-z

M3 - Journal article

SN - 0925-9864

VL - 58

SP - 657

EP - 666

JO - Genetic Resources and Crop Evolution

JF - Genetic Resources and Crop Evolution

IS - 5

ER -