A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

Claus Jeppesen; Kaj Frank Jensen; Peter E. Nielsen

A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

Claus Jeppesen, Kaj Frank Jensen, Peter E. Nielsen

Institut for Cellulær og Molekylær Medicin

7 Citationer (Scopus)

Abstract

Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.

Originalsprog	Engelsk
Tidsskrift	Nucleic Acids Research
Vol/bind	16
Udgave nummer	20
Sider (fra-til)	9545-9555
Antal sider	11
ISSN	0305-1048
Status	Udgivet - 25 okt. 1988

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC338762/pdf/nar00162-0176.pdf

Citationsformater

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title = "A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.",

abstract = "Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.",

keywords = "Cross-Linking Reagents, DNA/radiation effects, DNA-Directed RNA Polymerases/genetics, Escherichia coli/enzymology, Lac Operon/radiation effects, Photochemistry, Promoter Regions, Genetic/radiation effects, Templates, Genetic, Thymine/radiation effects, Transcription, Genetic/radiation effects, Ultraviolet Rays",

author = "Claus Jeppesen and Jensen, {Kaj Frank} and Nielsen, {Peter E.}",

year = "1988",

month = oct,

day = "25",

language = "English",

volume = "16",

pages = "9545--9555",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "20",

}

TY - JOUR

T1 - A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

AU - Jeppesen, Claus

AU - Jensen, Kaj Frank

AU - Nielsen, Peter E.

PY - 1988/10/25

Y1 - 1988/10/25

N2 - Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.

AB - Upon irradiation of the RNA polymerase-lacUV5 or deoP1 promoter complex with short wavelength ultraviolet light (lambda less than or equal to 300 nm) the polymerase is covalently crosslinked at an efficiency of greater than 10% to the first transcribed base of the template DNA strand when this is a thymine. The temperature dependence of this RNA polymerase-T+1 photoreaction strongly indicates a relation to the formation of the open complex. It is suggested that open complex formation is preceded or accompanied by a specific contact between the RNA polymerase and the first transcribed base of the DNA template.

KW - Cross-Linking Reagents

KW - DNA/radiation effects

KW - DNA-Directed RNA Polymerases/genetics

KW - Escherichia coli/enzymology

KW - Lac Operon/radiation effects

KW - Photochemistry

KW - Promoter Regions, Genetic/radiation effects

KW - Templates, Genetic

KW - Thymine/radiation effects

KW - Transcription, Genetic/radiation effects

KW - Ultraviolet Rays

M3 - Journal article

C2 - 3054808

SN - 0305-1048

VL - 16

SP - 9545

EP - 9555

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 20

ER -

A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

Abstract

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