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A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution
Aurelio Hidalgo, Anna Schliessmann,
Rafael Molina
, Juan Hermoso, Uwe T Bornscheuer
Protein Structure and Function Program
33
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Medicin og biovidenskab
Pseudomonas fluorescens
100%
Random Allocation
99%
Genetic Recombination
97%
Libraries
90%
Gene Order
87%
Esterases
81%
4-nitrophenyl dodecanoate
81%
Directed Molecular Evolution
64%
Protein Engineering
46%
Polymerase Chain Reaction
40%
Proteins
37%
Site-Directed Mutagenesis
36%
Oligonucleotides
35%
Mutagenesis
33%
Phenotype
20%
Mutation
18%
Teknik og materialevidenskab
Esterases
96%
Proteins
92%
Mutagenesis
80%
Genes
48%
Screening
47%
Gene encoding
45%
Oligonucleotides
37%
Scaffolds
29%
Substrates
17%
Kemiske forbindelser
Dodecanoate
52%
Protein
41%
Site-Directed Mutagenesis
35%
Oligonucleotide
29%
Mutation
28%
Time
12%