松仁蛋白酶解液体外抗氧化活性分析

Xiufang Lin, Xiaohong Wu, Zichen Zhao, Bingxin Xu, Xiaotang Gao, Min Liu

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    Abstract

    Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes ( pepsin and trypsin ) , and in vitro antioxidant activities of enzymatic
    hydrolysates of pine kernel protein obtained by hydrolysis of pepsin, trypsin and pepsin - trypsin were
    studied. The results showed that after enzymatic hydrolysis for 30 min, the pepsin enzymatic hydrolysate
    of pine kernel protein had the strongest in vitro antioxidant activity, and the scavenging rates on ·OH
    radical and O -
    2 ·radical were 67. 43% and 55. 66% respectively; the trypsin enzymatic hydrolysate of
    pine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for
    60 min,and the scavenging rate on ·OH radical was 68. 79% ,while it had the strongest scavenging capacity on O2- · radical after enzymatic hydrolysis for 120 min, and the scavenging rate on O2- · radical
    was 39. 20% ; pepsin - trypsin step - by - step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min,and the scavenging rates on·OH
    radical and O -
    2 ·radical were 79. 11% and 68. 99% respectively
    OriginalsprogKinesisk
    Tidsskrift中国油脂
    Vol/bind39
    Udgave nummer11
    Sider (fra-til)29-31
    Antal sider3
    ISSN1003-7969
    StatusUdgivet - 2014

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